|
| Status |
Public on Aug 19, 2009 |
| Title |
Whole embryo 3 |
| Sample type |
RNA |
| |
|
| Source name |
Xenopus Laevis Stage 15 whole embryo
|
| Organism |
Xenopus laevis |
| Characteristics |
Xenopus Laevis Stage 15 whole embryo
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Carried out at SUNY-MAC (Syracuse, NY) per manufacturers
protocol (Affymetrix).
|
| Label |
Biotin labelling
|
| Label protocol |
Carried out at SUNY-MAC (Syracuse, NY) per manufacturers
protocol (Affymetrix).
|
| |
|
| Hybridization protocol |
Carried out at SUNY-MAC (Syracuse, NY) per manufacturers
protocol (Affymetrix).
|
| Scan protocol |
Carried out at SUNY-MAC (Syracuse, NY) per manufacturers
protocol (Affymetrix).
|
| Description |
Carried out at SUNY-MAC (Syracuse, NY) per manufacturers
protocol (Affymetrix).
|
| Data processing |
GeneSpring GX, ver. 7.3.1 (Agilent Technologies) was used for analysis. Cel file
data was pre-processed using RMA method with the average signal intensity normalized to stage 15 whole embryos when analyzing EP, PNP and LE samples.
|
| |
|
| Submission date |
Sep 26, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Michael E Zuber |
| E-mail(s) |
zuberm@upstate.edu
|
| Organization name |
SUNY Upstate Medical University
|
| Department |
Ophthalmology
|
| Lab |
5329 Weiskotten Hall Addition
|
| Street address |
766 Irving Ave
|
| City |
Syracuse |
| State/province |
NY |
| ZIP/Postal code |
13202 |
| Country |
USA |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE9175 |
Eye primordia vs. posterior neural plate vs. lateral endoderm normalized the whole embryos |
|
