NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM231258 Query DataSets for GSM231258
Status Public on Oct 01, 2007
Title AV_HDM_2
Sample type RNA
 
Source name epithelial cell line, house dust mite exposed 24h
Organism Homo sapiens
Characteristics mucoepidermoid pulmonary carcinoma cell line NCI-H296
Treatment protocol cells were preincubated with HBSS 24 hours prior to adding stimulus, cells were exposed to stimulus for 24 hours.
Growth protocol Cells were cultured in RPMI1640 containing 10% FCS and penicillin.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
Description Gene expression data from cells after exposure to saline or HDM
Data processing The data were analyzed with Rosetta Resolver Software using default analysis settings. Data was normailzed using the rosetta normalization algorithm.
 
Submission date Sep 24, 2007
Last update date Aug 28, 2018
Contact name Aram Ben Vroling
E-mail(s) aramvroling@hotmail.com
Organization name AMC
Department ENT
Lab ENT
Street address meibergdreef 9
City Amsterdam
ZIP/Postal code 1100DD
Country Netherlands
 
Platform ID GPL570
Series (1)
GSE9151 Allergen induced gene expression of airway epithelial cells shows a possible role for TNF-α
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Rosetta normalized signal intensity

Data table
ID_REF VALUE
205922_at -4.83076
228558_at -10.36013
227182_at 1.11433
1562112_at 0.16557
227664_at -5.98702
228887_x_at -47.82162
231280_at -3.14203
215676_at -8.66821
219559_at -7.29166
204736_s_at -8.47797
238275_at 3.55543
238027_at -12.69172
241447_at -10.17679
231163_at 6.11591
211225_at -5.12447
223720_at -3.01243
215580_at 0.97168
214669_x_at -5.38167
237536_at 9.7781
220678_at -13.59526

Total number of rows: 54675

Table truncated, full table size 1058 Kbytes.




Supplementary file Size Download File type/resource
GSM231258.CEL.gz 4.7 Mb (ftp)(http) CEL
GSM231258.EXP.gz 326 b (ftp)(http) EXP
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap