|
| Status |
Public on Sep 01, 2008 |
| Title |
Primary peripheral blood B cell antiIgM -3hr - mAdbID:68980 |
| Sample type |
RNA |
| |
|
| Source name |
B cell antiIgM - 3 hour
|
| Organism |
Homo sapiens |
| Characteristics |
Cell type: Primary peripheral blood B cell
|
| Treatment protocol |
Treatment type: compound
Agent: anti_IgM
Treatment dose: 25ug/ml
Treatment time: 3 hour
Treatment temperature: 37C
In-vivo treatment: CD19+ peripheral blood B cells Rx w/ anti_IgM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Total RNA
Extraction method: Trizol
Other: Total RNA was extracted using Trizol according to manufacturer's instructions (Invitrogen, Carlsbad, CA).
|
| Label |
biotin
|
| Label protocol |
Affymetrix Labeling Protocol
Other: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microg mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Affymetrix Hybridization Protocol
Other: Following fragmentation, 20 micrograms of cRNA were hybridized for 16 hours at 45C on LymphDX Custom Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Affy Scanning Protocol
Other: Scanning was performed by the Affymetrix 3000 Scanner.
|
| Description |
mAdb experiment ID: 68980
|
| Data processing |
MAS 5.0 Data Processing Protocol
Calculation Method: The data were analyzed with Microarray Suite version 5.0 (MA S 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Sep 20, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Louis M. Staudt |
| E-mail(s) |
lstaudt@mail.nih.gov
|
| Phone |
301-402-1892
|
| Organization name |
National Cancer Institute
|
| Department |
Lymphoid Malignancies Branch
|
| Lab |
Louis M Staudt
|
| Street address |
9000 Rockville Pike, Bldg 10, Rm 4N114
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9119 |
Primary human peripheral Blood B cell Acute Stimulation - anti_IgM |
|
| Relations |
| Reanalyzed by |
GSE119087 |
