|
Status |
Public on Jul 07, 2008 |
Title |
KMS12 day4a shIRF4b - repeat 1 - mAdbID:87191 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
KMS12 shi_2046 Untreated
|
Organism |
Homo sapiens |
Characteristics |
Cell line: KMS12 Cell type: myeloma Disease state: myeloma genotype/variation: shirf4_2046 (shIRFb)
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol Total RNA Extraction method: Trizol Other: Total RNA was extracted using Trizol according to manufacturer's instructions (Invitrogen, Carlsbad, CA).
|
Label |
cy3
|
Label protocol |
Agilent Labeling-Cy3 Other: Total RNA was reverse transcribed to cDNA using T7 Promoter Primer and MMLV-RT. Then the cDNA was converted to aRNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3- or cyanine 5-labeled CTP (Two-Color Microarray-Based Gene Expression Analysis Protocol, Agilent).
|
|
|
Channel 2 |
Source name |
KMS12 shi_2046 day4
|
Organism |
Homo sapiens |
Characteristics |
Cell line: KMS12 Cell type: myeloma Disease state: myeloma genotype/variation: shirf4_2046 (shIRFb)
|
Treatment protocol |
Treatment type: compound Agent: doxycycline Treatment dose: 20 ng/ml Treatment time: 4 days Treatment temperature: 37c
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol Total RNA Extraction method: Trizol Other: Total RNA was extracted using Trizol according to manufacturer's instructions (Invitrogen, Carlsbad, CA).
|
Label |
cy5
|
Label protocol |
Agilent Labeling-Cy5 Other: Total RNA was reverse transcribed to cDNA using T7 Promoter Primer and MMLV-RT. Then the cDNA was converted to aRNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3- or cyanine 5-labeled CTP (Two-Color Microarray-Based Gene Expression Analysis Protocol, Agilent).
|
|
|
|
Hybridization protocol |
Agilent Hybridization Other: According to the manufacture's recommended protocol (Two-Color Microarray-Based Gene Expression Analysis, Agilent).
|
Scan protocol |
Agilent Scanning Protocol Other: Arrays were scanned at 5um resolution on an Agilent DNA Microarray Scanner (G2505B, Agilent) using the default settings for 4x44k format two-color arrays.
|
Description |
mAdb experiment ID: 87191
|
Data processing |
Agilent Data Processing Protocol Calculation Method: Images were auto gridded, analyzed and data extracted using Agilent Feature Extraction Software (Version 9.1.3.1). Spot values were normalized using the default linear-lowess normalization.
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|
|
Submission date |
Sep 17, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Louis M. Staudt |
E-mail(s) |
lstaudt@mail.nih.gov
|
Phone |
301-402-1892
|
Organization name |
National Cancer Institute
|
Department |
Lymphoid Malignancies Branch
|
Lab |
Louis M Staudt
|
Street address |
9000 Rockville Pike, Bldg 10, Rm 4N114
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE9067 |
Myeloma IRF4 target genes - Agilent |
|