|
| Status |
Public on Sep 30, 2007 |
| Title |
SHED098 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Pool
|
| Organism |
Homo sapiens |
| Characteristics |
Pool of human subcutaneous adipose tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from scWAT was prepared using the RNeasy RNA Mini Kit (Qiagen, Courtaboeuf, France).
|
| Label |
Cy3
|
| Label protocol |
One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion, Austin, TX), and 3 µg aRNA was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences, Orsay, France) (available at http://cmgm.stanford.edu/pbrown/protocols/index).
|
| |
|
| Channel 2 |
| Source name |
Obese
|
| Organism |
Homo sapiens |
| Characteristics |
Obese subcutaneous adipose tissue (ZEAU)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from scWAT was prepared using the RNeasy RNA Mini Kit (Qiagen, Courtaboeuf, France).
|
| Label |
Cy5
|
| Label protocol |
One microgram of total RNA from each sample preparation was amplified using the MessageAmp RNA kit (Ambion, Austin, TX), and 3 µg aRNA was Cy-dye labeled using the CyScribe first-strand cDNA labeling kit (Amersham Biosciences, Orsay, France) (available at http://cmgm.stanford.edu/pbrown/protocols/index).
|
| |
|
| |
| Hybridization protocol |
The labeled cDNA mixtures were hybridized according to the protocol described at http://cmgm.stanford.edu/pbrown/protocols/index
|
| Scan protocol |
The arrays were immediately scanned using a GenePix 4000A confocal Scanner (Axon Instruments). Images were analyzed using GenePix pro 3 software.
|
| Description |
We analyzed the gene expression profiles of subcutaneous white adipose tissue from 29 obese subjects during very low calorie diet (VLCD) using cDNA microarray. The patterns of expression were compared with that of 17 non-obese subjects.
|
| Data processing |
After scanning the arrays, the images were analyzed and ratio intensities were filtered and normalized using Stanford Microarray Database Online resources (http://genome-www5.stanford.edu/).The VALUE field represents the log(base2) of the print-tip loess normalized R/G ratio (mean).
|
| |
|
| Submission date |
Sep 12, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Corneliu Henegar |
| E-mail(s) |
corneliu@henegar.info, flavien.jacques@upmc.fr
|
| Organization name |
Institut national de la santé et de la recherche médicale
|
| Department |
U872 Eq7
|
| Lab |
Eq7 Nutriomique
|
| Street address |
15 Rue de l'Ecole de Medecine
|
| City |
Paris |
| State/province |
Ile de France |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL3942 |
| Series (2) |
| GSE9153 |
Expression profiling of human adipose tissue in obese subjects |
| GSE9157 |
Expression profiling of human adipose tissue in obese and lean subjects and in various clinical conditions |
|
