|
| Status |
Public on Dec 27, 2007 |
| Title |
BP.3 |
| Sample type |
RNA |
| |
|
| Source name |
distal small intestine from Rag 1
|
| Organism |
Mus musculus |
| Characteristics |
Rag 1-/- mouse with 225.4 anti-B.thetaiotaomicron specific IgA producing hybridoma growing subcutaneously colonized with B. thetaiotaomicron for 10 days.
|
| Growth protocol |
Distal Small intestine (Whole intestinal segments 10, 11, 12, and 14 pooled) from mice colonized for 10 days with B. thetaiotaomicron.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
to be added
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 40 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse 430.2. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GS300
|
| Description |
Comparison of Wildtype B6 and Rag1 mice colonized for 10 days with Bacteroides thetaiotaomicron
|
| Data processing |
Image files were analyzed in dCHIP06, samples were normalized to the B6.2 chip, using the quartile method.
|
| |
|
| Submission date |
Sep 12, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Daniel A Peterson |
| Phone |
314 362 5443
|
| Organization name |
Center for Genome Sciences
|
| Department |
Molecular Biology and Pharmacology
|
| Lab |
Jeffrey I Gordon
|
| Street address |
4444 Forest Park
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63021 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE9018 |
IgA impact on distal small intestine after colonization with B. thetaiotaomicron |
|
| Relations |
| Reanalyzed by |
GSE119085 |
