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Status
Public on May 21, 2004
Title
B chip Version2: time 15 day Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 15 day Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
166.27
114.419
139.446
133.955
85.5
44
72.5
43
2
-1.43E-01
0
176.8
139.055
161.858
122.942
101
71
75
46
3
3.28E-01
0
975.246
172.993
840.827
147.071
840
91
703
81
4
2.89E-01
0
194.609
168.103
164.522
132.737
99
81
78
71
5
1.48E-01
0
980.807
173.202
896.847
133.289
870
112
708
60
6
2.72E-01
0
415.585
129.448
398.194
130.328
316
38
284
61
7
5.54E-01
0
251.12
146.407
209.423
160.038
155
62
131
77.5
8
5.84E-01
0
219.353
126.382
199.05
143.147
143
53
101
86.5
9
-9.48E-02
0
287.092
151.853
275.145
135.318
208
72.5
186
49
10
-3.91E-02
0
184.839
143.374
157.166
119.743
109.5
78.5
81
48.5
11
-8.95E-02
0
184.709
173.481
177.77
161.244
92
90
111
78
12
1.30E-01
0
183.92
151.903
151.627
155.195
105
91.5
66
66.5
13
-5.02E-01
0
218.931
179.97
199.655
118.821
130
98.5
105
34
14
1.08E-01
0
191.797
135.6
155.793
123.255
116
69
73
42
15
3.79E-01
0
427.297
159.62
384.299
173.125
330
82
281
107
16
-5.03E-01
0
257.327
189.954
266.349
165.546
177
124.5
187.5
82
17
2.58E-01
0
426.352
145.244
405.475
155.477
336
55
294
72
18
1.70E-01
0
197.513
158.125
163.698
140.738
114
83.5
92
62
19
-2.22E-01
0
203.582
154.048
196.639
142.215
132.5
87
104.5
63
20
-6.27E-02
0
163.155
136.325
165.8
111.03
74
47
89
45.5
Total number of rows: 14720 Table truncated, full table size 924 Kbytes .Supplementary data files not provided