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Sample GSM22865 Query DataSets for GSM22865
Status Public on May 21, 2004
Title B chip Version2: time 15 day Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 15 day Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 166.27 114.419 139.446 133.955 85.5 44 72.5 43
2 -1.43E-01 0 176.8 139.055 161.858 122.942 101 71 75 46
3 3.28E-01 0 975.246 172.993 840.827 147.071 840 91 703 81
4 2.89E-01 0 194.609 168.103 164.522 132.737 99 81 78 71
5 1.48E-01 0 980.807 173.202 896.847 133.289 870 112 708 60
6 2.72E-01 0 415.585 129.448 398.194 130.328 316 38 284 61
7 5.54E-01 0 251.12 146.407 209.423 160.038 155 62 131 77.5
8 5.84E-01 0 219.353 126.382 199.05 143.147 143 53 101 86.5
9 -9.48E-02 0 287.092 151.853 275.145 135.318 208 72.5 186 49
10 -3.91E-02 0 184.839 143.374 157.166 119.743 109.5 78.5 81 48.5
11 -8.95E-02 0 184.709 173.481 177.77 161.244 92 90 111 78
12 1.30E-01 0 183.92 151.903 151.627 155.195 105 91.5 66 66.5
13 -5.02E-01 0 218.931 179.97 199.655 118.821 130 98.5 105 34
14 1.08E-01 0 191.797 135.6 155.793 123.255 116 69 73 42
15 3.79E-01 0 427.297 159.62 384.299 173.125 330 82 281 107
16 -5.03E-01 0 257.327 189.954 266.349 165.546 177 124.5 187.5 82
17 2.58E-01 0 426.352 145.244 405.475 155.477 336 55 294 72
18 1.70E-01 0 197.513 158.125 163.698 140.738 114 83.5 92 62
19 -2.22E-01 0 203.582 154.048 196.639 142.215 132.5 87 104.5 63
20 -6.27E-02 0 163.155 136.325 165.8 111.03 74 47 89 45.5

Total number of rows: 14720

Table truncated, full table size 924 Kbytes.




Supplementary data files not provided

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