NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22864 Query DataSets for GSM22864
Status Public on May 21, 2004
Title B chip Version2: time 15 day Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 15 day Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 205.291 182.705 178.035 154.232 129 122.5 97.5 71.5
2 -6.59E-01 0 229.852 212.086 228.084 185.812 150 158 161 108
3 2.26E-01 0 763.204 240.149 836.464 204.951 639 157 665 129
4 NULL 0 232.564 169.867 190.51 193.104 172 96 118 118
5 2.57E-01 0 687.375 219.34 759.137 157.119 607 98 617 89
6 1.47E-01 0 382.197 183.332 418.494 157.943 327 94.5 334 93.5
7 1.48E-01 0 256.958 195.848 239.865 172.485 171 121 170 99
8 3.15E-01 0 222.715 173.552 222.637 187.79 148 93.5 143 106.5
9 -4.75E-03 0 304.913 190.962 344.077 174.25 231 114 258 124
10 6.63E-01 0 203.792 185.03 207.955 215.603 130 103 142 144
11 -2.85E-01 0 189.051 202.757 198.927 197.125 112.5 127 120 124
12 -7.21E-02 0 188.784 186.798 180.89 139.506 107 88 103 67
13 -9.81E-02 0 263.42 205.196 242.33 165.685 198 135.5 151 84.5
14 1.12E-01 0 216.696 192.426 206.352 186.27 139 115.5 123 108
15 -3.09E-01 0 308.323 190.636 335.501 165.746 211 133 231 84
16 -5.24E-01 0 218.437 207.944 227.626 164.124 155 132.5 156 93.5
17 -9.35E-02 0 356.616 206.832 395.622 189.765 260 129.5 317 106
18 5.62E-01 0 228.941 173.335 202.014 194.417 147 106 147 114.5
19 1.50E-01 0 254.543 211.244 231.772 172.718 169 117.5 158 90
20 1.23E-01 0 194.012 176.777 206.76 203.027 117 103.5 134 121

Total number of rows: 14720

Table truncated, full table size 942 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap