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Status
Public on May 21, 2004
Title
B chip Version2: time 15 day Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 15 day Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
205.291
182.705
178.035
154.232
129
122.5
97.5
71.5
2
-6.59E-01
0
229.852
212.086
228.084
185.812
150
158
161
108
3
2.26E-01
0
763.204
240.149
836.464
204.951
639
157
665
129
4
NULL
0
232.564
169.867
190.51
193.104
172
96
118
118
5
2.57E-01
0
687.375
219.34
759.137
157.119
607
98
617
89
6
1.47E-01
0
382.197
183.332
418.494
157.943
327
94.5
334
93.5
7
1.48E-01
0
256.958
195.848
239.865
172.485
171
121
170
99
8
3.15E-01
0
222.715
173.552
222.637
187.79
148
93.5
143
106.5
9
-4.75E-03
0
304.913
190.962
344.077
174.25
231
114
258
124
10
6.63E-01
0
203.792
185.03
207.955
215.603
130
103
142
144
11
-2.85E-01
0
189.051
202.757
198.927
197.125
112.5
127
120
124
12
-7.21E-02
0
188.784
186.798
180.89
139.506
107
88
103
67
13
-9.81E-02
0
263.42
205.196
242.33
165.685
198
135.5
151
84.5
14
1.12E-01
0
216.696
192.426
206.352
186.27
139
115.5
123
108
15
-3.09E-01
0
308.323
190.636
335.501
165.746
211
133
231
84
16
-5.24E-01
0
218.437
207.944
227.626
164.124
155
132.5
156
93.5
17
-9.35E-02
0
356.616
206.832
395.622
189.765
260
129.5
317
106
18
5.62E-01
0
228.941
173.335
202.014
194.417
147
106
147
114.5
19
1.50E-01
0
254.543
211.244
231.772
172.718
169
117.5
158
90
20
1.23E-01
0
194.012
176.777
206.76
203.027
117
103.5
134
121
Total number of rows: 14720 Table truncated, full table size 942 Kbytes .Supplementary data files not provided