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Status
Public on May 21, 2004
Title
B chip Version2: time 5 day Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
165.512
188.151
188.639
188.335
80
95
109
118
2
1.32E-01
0
194.08
164.217
223.664
209.175
121.5
105.5
137.5
132
3
4.23E-01
0
480.237
181.131
530.266
205.861
393
110.5
417
160
4
2.54E-01
0
202.787
180.9
243.383
189.8
116
86
169
131.5
5
-2.06E-01
0
477.932
167.727
702.935
167.471
403
101
558
114.5
6
-1.90E-01
0
296.08
208.216
378.2
195.591
196
120.5
281.5
127
7
1.82E-01
0
256.789
184.985
267.366
201.463
166
110.5
179.5
115
8
-1.50E-01
0
218.942
182.084
243.764
180.965
152
113.5
166.5
108
9
8.36E-02
0
241.116
170.582
287.202
197.236
168
115.5
216
149.5
10
2.27E-01
0
196.424
190.609
206.385
176.434
117
100
143
113.5
11
3.79E-01
0
174.583
138.19
209.998
194.374
99
58
151
109
12
-5.75E-01
0
182.748
195.935
196.657
184.734
109
127
121
103
13
3.82E-02
0
211.182
156.739
281.37
188.514
118
75
181
125
14
-5.93E-01
0
188.37
190.615
231.594
214.648
109
127
163
126
15
-1.90E-01
0
275.543
186.631
377.556
220.772
194
109.5
311
143.5
16
-5.22E-01
0
197.701
183.576
254.454
192.54
134
114.5
199
119
17
2.10E-01
0
260.655
166.08
362.399
216.255
177.5
68.5
286
157.5
18
-6.36E-01
0
189.414
175.003
259.245
201.825
112
114
194
126.5
19
3.71E-01
0
206.218
190.559
228.455
225.267
133
104
164
139.5
20
-5.99E-01
0
180.455
176.747
291.995
245.435
108.5
110
237
170.5
Total number of rows: 14720 Table truncated, full table size 947 Kbytes .Supplementary data files not provided