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Sample GSM22860 Query DataSets for GSM22860
Status Public on May 21, 2004
Title B chip Version2: time 5 day Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 165.512 188.151 188.639 188.335 80 95 109 118
2 1.32E-01 0 194.08 164.217 223.664 209.175 121.5 105.5 137.5 132
3 4.23E-01 0 480.237 181.131 530.266 205.861 393 110.5 417 160
4 2.54E-01 0 202.787 180.9 243.383 189.8 116 86 169 131.5
5 -2.06E-01 0 477.932 167.727 702.935 167.471 403 101 558 114.5
6 -1.90E-01 0 296.08 208.216 378.2 195.591 196 120.5 281.5 127
7 1.82E-01 0 256.789 184.985 267.366 201.463 166 110.5 179.5 115
8 -1.50E-01 0 218.942 182.084 243.764 180.965 152 113.5 166.5 108
9 8.36E-02 0 241.116 170.582 287.202 197.236 168 115.5 216 149.5
10 2.27E-01 0 196.424 190.609 206.385 176.434 117 100 143 113.5
11 3.79E-01 0 174.583 138.19 209.998 194.374 99 58 151 109
12 -5.75E-01 0 182.748 195.935 196.657 184.734 109 127 121 103
13 3.82E-02 0 211.182 156.739 281.37 188.514 118 75 181 125
14 -5.93E-01 0 188.37 190.615 231.594 214.648 109 127 163 126
15 -1.90E-01 0 275.543 186.631 377.556 220.772 194 109.5 311 143.5
16 -5.22E-01 0 197.701 183.576 254.454 192.54 134 114.5 199 119
17 2.10E-01 0 260.655 166.08 362.399 216.255 177.5 68.5 286 157.5
18 -6.36E-01 0 189.414 175.003 259.245 201.825 112 114 194 126.5
19 3.71E-01 0 206.218 190.559 228.455 225.267 133 104 164 139.5
20 -5.99E-01 0 180.455 176.747 291.995 245.435 108.5 110 237 170.5

Total number of rows: 14720

Table truncated, full table size 947 Kbytes.




Supplementary data files not provided

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