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Status
Public on May 21, 2004
Title
B chip Version2: time 5 day Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
153.412
148.576
227.643
176.76
75
71
159
96
2
-1.01E+00
0
211.578
185.239
333.155
213.607
115
113
260
113
3
2.18E-01
0
1747.87
182.663
2121.16
247.946
1438
118.5
1846
201.5
4
-1.84E-01
0
182.258
165.163
265.318
238.24
94
93.5
177.5
156.5
5
-1.59E-01
0
1042.01
141.764
1527.74
171.375
907
59.5
1273
112
6
6.59E-01
0
803.914
175.823
727.778
211.717
693
93
634
131
7
-2.87E-01
0
284.641
163.23
418.889
220.566
216
88
328
146
8
-4.35E-01
0
233.635
173.613
335.734
190.257
141
90
259
110
9
NULL
0
574.554
165.015
486.164
169.896
470
93
399
92.5
10
-8.05E-01
0
166.361
162.179
264.594
170.29
80.5
81.5
184.5
106
11
9.39E-02
0
191.05
134.245
253.753
186.313
111.5
52.5
186
105
12
NULL
0
198.304
159.738
228.111
160.781
106
60
133
91
13
3.53E-01
0
349.166
180.188
373.118
220.659
283
105
276
150
14
-1.44E-01
0
203.806
171.33
294.994
205.708
127
84
215
147
15
-4.86E-02
0
750
179.101
992.557
208.477
635
91
814
121
16
-8.58E-01
0
326.255
175.333
616.607
204.733
247
100
455
130
17
2.87E-01
0
1038.42
212.259
1170.79
277.71
939
131
898
207
18
5.61E-01
0
358.285
160.854
389.696
223.246
283
86
295
172
19
-4.22E-02
0
305.942
188.022
344.515
174.319
234
99.5
249
100.5
20
-4.08E-01
0
213.497
191.646
279.569
200.258
130.5
105.5
203.5
121.5
Total number of rows: 14720 Table truncated, full table size 942 Kbytes .Supplementary data files not provided