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Status
Public on May 21, 2004
Title
B chip Version2: time 3 day Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 day Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
241.923
188.172
196.894
219.228
131
97
102
112
2
2.13E-01
0
301.252
193.2
233.169
193.98
192
125.5
118
104.5
3
-9.79E-01
0
1321.09
313.799
1502.15
233.317
1148
225
1271
112
4
-2.39E-01
0
334.849
290.031
259.05
182.012
215.5
180.5
127
106
5
4.85E-01
0
3618.35
303.851
1595.77
263.74
3497
182
1440
148
6
3.81E-01
0
1036.83
238.551
604.608
242.541
937
135
493
130
7
7.61E-01
0
907.197
260.261
425.197
200.275
711
144
263
103
8
-3.54E-02
0
430.964
238.912
343.379
213.459
290
124
200
114
9
5.51E-01
0
837.517
262.073
443.51
215.46
719
157
282
110
10
5.46E-01
0
308.492
242.97
218.286
212.312
191
119
114
106.5
11
2.04E-01
0
299.958
236.643
225.715
193.873
175.5
130.5
110.5
99.5
12
-1.72E-01
0
283.459
233.121
234.272
200.97
171.5
154
120
103
13
4.80E-01
0
465.13
245.489
268.124
181.511
312
135
145
93
14
3.47E-02
0
293.985
249.313
239.051
194.587
169
123
113
99
15
3.58E-01
0
783.257
254.1
453.632
182.955
637
142
285
95
16
-6.56E-01
0
371.234
256.241
347.416
187.904
209
155
202.5
95.5
17
-1.30E-01
0
735.996
344.606
584.339
269.31
569
198
482
163
18
-1.69E-01
0
293.131
238.572
274.842
232.057
155
119
143
115
19
9.91E-02
0
319.861
211.811
253.034
183.196
200
126.5
135
106
20
1.53E-01
0
360.671
225.011
269.526
198.281
210
115
149
100
Total number of rows: 14720 Table truncated, full table size 952 Kbytes .Supplementary data files not provided