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Sample GSM22853 Query DataSets for GSM22853
Status Public on May 21, 2004
Title B chip Version2: time 3 day Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 day Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 241.923 188.172 196.894 219.228 131 97 102 112
2 2.13E-01 0 301.252 193.2 233.169 193.98 192 125.5 118 104.5
3 -9.79E-01 0 1321.09 313.799 1502.15 233.317 1148 225 1271 112
4 -2.39E-01 0 334.849 290.031 259.05 182.012 215.5 180.5 127 106
5 4.85E-01 0 3618.35 303.851 1595.77 263.74 3497 182 1440 148
6 3.81E-01 0 1036.83 238.551 604.608 242.541 937 135 493 130
7 7.61E-01 0 907.197 260.261 425.197 200.275 711 144 263 103
8 -3.54E-02 0 430.964 238.912 343.379 213.459 290 124 200 114
9 5.51E-01 0 837.517 262.073 443.51 215.46 719 157 282 110
10 5.46E-01 0 308.492 242.97 218.286 212.312 191 119 114 106.5
11 2.04E-01 0 299.958 236.643 225.715 193.873 175.5 130.5 110.5 99.5
12 -1.72E-01 0 283.459 233.121 234.272 200.97 171.5 154 120 103
13 4.80E-01 0 465.13 245.489 268.124 181.511 312 135 145 93
14 3.47E-02 0 293.985 249.313 239.051 194.587 169 123 113 99
15 3.58E-01 0 783.257 254.1 453.632 182.955 637 142 285 95
16 -6.56E-01 0 371.234 256.241 347.416 187.904 209 155 202.5 95.5
17 -1.30E-01 0 735.996 344.606 584.339 269.31 569 198 482 163
18 -1.69E-01 0 293.131 238.572 274.842 232.057 155 119 143 115
19 9.91E-02 0 319.861 211.811 253.034 183.196 200 126.5 135 106
20 1.53E-01 0 360.671 225.011 269.526 198.281 210 115 149 100

Total number of rows: 14720

Table truncated, full table size 952 Kbytes.




Supplementary data files not provided

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