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Status
Public on May 21, 2004
Title
B chip Version2: time 3 day Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 day Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
194.65
157.648
245.576
249.799
113
88
187
172
2
NULL
0
315.37
190.611
367.475
258.183
227
105
286
175
3
-9.46E-01
0
1547.92
236.724
2460.68
307.917
1280
166.5
1896
231
4
-2.76E-01
0
293.056
200.03
345.019
225.76
225
115
276
154
5
3.60E-01
0
4543.46
234.387
2852.65
289.308
4197
161
2486
227
6
1.37E-02
0
982.182
205.503
952.328
263.213
853
114.5
765
182
7
7.94E-01
0
572.942
200.892
418.73
251.673
455
135
340
192
8
-3.18E-01
0
398.721
221.698
424.541
236.846
288
130.5
330
141
9
1.56E-01
0
738.113
208.447
648.754
234.92
652
134
522
161
10
-3.41E-02
0
295.721
199.21
310.748
238.367
198
109
234
138.5
11
3.12E-01
0
293.582
162.365
314.056
226.64
199
88
252
162
12
2.98E-01
0
246.098
176.475
288.903
253.184
174
98
198
176
13
1.43E-01
0
401.82
195.668
382.562
203.118
326.5
108
274.5
151.5
14
6.51E-03
0
266.63
198.241
290.27
231.818
180
112
227
148
15
1.26E-01
0
980.678
179.642
892.77
235.052
887
103
719
167
16
-2.83E-01
0
393.548
177.634
463.887
227.403
332
105
375
165.5
17
-8.48E-02
0
908.913
230.106
924.652
281.031
765
153
727
205
18
-5.50E-01
0
279.415
245.49
328.027
246.608
195
152
246
160
19
-6.14E-01
0
282.61
208.873
353.257
237.124
207
142.5
253
162
20
2.46E-01
0
274.431
198
296.577
221.459
201
96
220
156.5
Total number of rows: 14720 Table truncated, full table size 954 Kbytes .Supplementary data files not provided