|
| Status |
Public on Aug 19, 2016 |
| Title |
Mu50-1 |
| Sample type |
RNA |
| |
|
| Source name |
Mu50 wild-type
|
| Organism |
Staphylococcus aureus subsp. aureus Mu50 |
| Characteristics |
genotype/variation: wild type
|
| Treatment protocol |
The knockout mutant strains generated by allelic exchange method.
|
| Growth protocol |
Cells were grown exponentially to an O.D. 600nm of approximately 0.5, then harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Rneasy Mini Kit (Qiagen) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA quality and concentration was determined by analysis with an Agilent 2100 bioanalyzer at the Harvard-Partners Center for Genetics and Genomics (Harvard Medical School, Cambridge, MA USA).
|
| Label |
Cy3
|
| Label protocol |
cDNA were prepared from 10 ug total RNA using the SuperScript double-stranded cDNA synthesis kit(Invitrogen). Labeling were carried out according to NimbleGen protocols.
|
| |
|
| Hybridization protocol |
Hybridization were carried out according to NimbleGen protocols.
|
| Scan protocol |
Arrays were scanned using the NimbleGen MS 200 Microarray Scanner
|
| Description |
N_Mu50-1
This sample is of wild-type Staphylococcus aureus Mu50. It is the first of four wild-type biological replicates used in this experiment, each from separate cultures.
|
| Data processing |
Array data export processing and analysis was performed using NimbleScan v2.5 (RMA algorithm)
|
| |
|
| Submission date |
Aug 18, 2016 |
| Last update date |
Aug 19, 2016 |
| Contact name |
Jung Wook Kim |
| E-mail(s) |
jungwookk@naver.com
|
| Organization name |
Korea National Research Institute of Health
|
| Department |
Division of Antimicrobial resistance
|
| Street address |
Osong Health Technology Administration Complex, 187
|
| City |
Cheongju |
| ZIP/Postal code |
363700 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL22334 |
| Series (1) |
| GSE85824 |
Expression analysis of Staphylococcus aureus Mu50 delta-SAV1322 mutant |
|
