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Status
Public on May 21, 2004
Title
B chip Version2: time 3 day Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 day Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
206.781
189.311
179.288
201.746
138.5
120
93.5
110.5
2
NULL
0
245.386
237.302
217.399
176.776
182
173.5
141
112
3
-4.99E-01
0
335.851
221.364
611.159
221.578
276.5
154
513
145.5
4
NULL
0
234.612
195.56
239.791
199.193
153
129
172
143
5
5.84E-01
0
1865.12
264.686
2808.27
217.236
1784
223
2698
164
6
NULL
0
315.08
202.969
368.585
227.459
263
133
295
180
7
4.50E-01
0
6656.91
284.683
10086.3
318.764
7002
190
10617
259
8
NULL
0
256.056
222.922
241.603
214.612
191
159
163
129
9
3.39E-01
0
281.567
189.764
290.289
178.682
208.5
112.5
222.5
106
10
NULL
0
248.009
228.254
199.385
172.589
176.5
166
107.5
111
11
NULL
0
224.865
211.976
195.1
216.436
154
161
116
133
12
NULL
0
241.321
205.831
198.153
186.803
159
142
129
112
13
NULL
0
282.541
271.062
311.142
211.133
220
216
241
143
14
NULL
0
234.45
227.3
191.114
202.245
165
134
110
115
15
-1.45E-01
0
330.06
210.024
453.971
205.638
278
152
380
125
16
2.32E-01
0
279.582
229.569
246.548
179.324
215.5
141
159
107
17
6.00E-02
0
394.843
259.863
496.666
201.236
311
190
420
145.5
18
4.54E-01
0
299.667
232.324
299.996
228.228
248
143
221
158
19
-3.46E-02
0
276.758
243.44
234.572
169.164
196
156.5
147
95.5
20
-3.83E-01
0
284.736
218.851
236.301
171.882
215
178
161
82
Total number of rows: 14720 Table truncated, full table size 938 Kbytes .Supplementary data files not provided