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Status
Public on May 21, 2004
Title
B chip Version2: time 24 hour Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
239.936
219.452
297.454
267.106
164
154
247
199
2
NULL
0
326.614
265.204
380.011
322.993
258
185.5
325
263
3
-5.04E-02
0
3324.19
371.748
5788.75
554.265
3345
301.5
5700
483
4
-3.03E-01
0
389.856
260.209
581.002
360.787
307
199.5
507
276.5
5
-3.13E-02
0
9956.03
352.867
15007.2
463.843
10738
283
15860
412
6
1.23E-01
0
1359.72
274.754
2214.72
520.779
1204
199.5
2023
479.5
7
-4.35E-01
0
26525.3
459.631
31476.7
920.449
29881
404
34511
899
8
-1.78E-01
0
441.098
311.887
628.825
431.775
349
270
533
390
9
5.05E-01
0
659.784
224.335
774.39
304.441
533
141
648
253
10
NULL
0
252.747
209.115
313.915
290.445
181
143
245
223.5
11
NULL
0
247.796
221.562
279.103
274.646
160.5
155.5
210.5
197
12
NULL
0
288.779
250.063
372.649
266.966
219
168
294.5
161.5
13
7.89E-01
0
1253.88
213.963
1335.87
369.859
1122
159
1164
313
14
NULL
0
251.246
234.143
351.447
287.398
194
170
304
226
15
7.12E-02
0
1080.18
260.069
1696.02
387.363
963
201
1516
361
16
-3.24E-01
0
447.197
290.705
815.446
489.342
377
230
755
456
17
-3.42E-02
0
1775.89
456.616
2963.28
767.925
1685
388
2656
627
18
-2.26E-01
0
766.259
273.846
1361.77
422.243
653
201
1183
370
19
-5.26E-01
0
327.379
240.147
606.22
359.578
267
162
518
304
20
NULL
0
306.694
199.864
567.059
430.434
234.5
118.5
509.5
394.5
Total number of rows: 14720 Table truncated, full table size 954 Kbytes .Supplementary data files not provided