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Sample GSM22847 Query DataSets for GSM22847
Status Public on May 21, 2004
Title B chip Version2: time 24 hour Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 239.936 219.452 297.454 267.106 164 154 247 199
2 NULL 0 326.614 265.204 380.011 322.993 258 185.5 325 263
3 -5.04E-02 0 3324.19 371.748 5788.75 554.265 3345 301.5 5700 483
4 -3.03E-01 0 389.856 260.209 581.002 360.787 307 199.5 507 276.5
5 -3.13E-02 0 9956.03 352.867 15007.2 463.843 10738 283 15860 412
6 1.23E-01 0 1359.72 274.754 2214.72 520.779 1204 199.5 2023 479.5
7 -4.35E-01 0 26525.3 459.631 31476.7 920.449 29881 404 34511 899
8 -1.78E-01 0 441.098 311.887 628.825 431.775 349 270 533 390
9 5.05E-01 0 659.784 224.335 774.39 304.441 533 141 648 253
10 NULL 0 252.747 209.115 313.915 290.445 181 143 245 223.5
11 NULL 0 247.796 221.562 279.103 274.646 160.5 155.5 210.5 197
12 NULL 0 288.779 250.063 372.649 266.966 219 168 294.5 161.5
13 7.89E-01 0 1253.88 213.963 1335.87 369.859 1122 159 1164 313
14 NULL 0 251.246 234.143 351.447 287.398 194 170 304 226
15 7.12E-02 0 1080.18 260.069 1696.02 387.363 963 201 1516 361
16 -3.24E-01 0 447.197 290.705 815.446 489.342 377 230 755 456
17 -3.42E-02 0 1775.89 456.616 2963.28 767.925 1685 388 2656 627
18 -2.26E-01 0 766.259 273.846 1361.77 422.243 653 201 1183 370
19 -5.26E-01 0 327.379 240.147 606.22 359.578 267 162 518 304
20 NULL 0 306.694 199.864 567.059 430.434 234.5 118.5 509.5 394.5

Total number of rows: 14720

Table truncated, full table size 954 Kbytes.




Supplementary data files not provided

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