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Status
Public on May 21, 2004
Title
B chip Version2: time 8 hour Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
207.453
207.411
183.632
145.737
131.5
136
109
60
2
-0.115595119
0
256.077
226.419
180.334
169.906
180.5
163.5
116
96
3
0.862953395
0
2362.95
347.105
1027.9
287.764
2251
263
920
236
4
0.092488757
0
424.004
263.333
267.2
194.196
352
185.5
207
110.5
5
0.466867579
0
10459.3
307.023
4310.14
249.528
11158
253
4303
197
6
0.460607503
0
1062.81
268.847
581.678
231.466
889
180
513
181
7
-0.118948057
0
23004
474.366
8999.72
336.564
25480
392
9934
281
8
1.525603295
0
432.922
264.962
235.787
229.548
361
162.5
186
159.5
9
-0.067695336
0
490.57
203.802
353.051
179.226
414
135
271
97
10
0.3100333
0
263.206
201.014
172.319
164.78
192
137
104
89
11
NULL
0
216.941
210.183
175.388
140.381
145
148
99
67.5
12
-0.464193455
0
266.994
192.826
207.908
151.078
187
140
120.5
78
13
1.00019615
0
1147.27
207.077
398.233
143.161
1009
111.5
295
64
14
-0.090917522
0
228.77
172.556
184.755
142.479
165.5
93
91.5
74
15
0.126912731
0
791.654
260.471
451.956
179.48
679
208
356
103.5
16
-0.241543838
0
388.96
264.339
298.867
235.315
326
215.5
217
152.5
17
-0.18910632
0
1104.26
403.032
749.874
332.558
965
344
646
232
18
0.486778044
0
670.758
251.233
411.251
232.233
556
192
352
170
19
-0.113507272
0
333.74
219.14
233.392
179.627
256
160
161
97
20
0.158798697
0
408.038
289.316
240.554
177.847
329
226.5
159
119.5
Total number of rows: 14720 Table truncated, full table size 992 Kbytes .Supplementary data files not provided