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Sample GSM22846 Query DataSets for GSM22846
Status Public on May 21, 2004
Title B chip Version2: time 8 hour Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 207.453 207.411 183.632 145.737 131.5 136 109 60
2 -0.115595119 0 256.077 226.419 180.334 169.906 180.5 163.5 116 96
3 0.862953395 0 2362.95 347.105 1027.9 287.764 2251 263 920 236
4 0.092488757 0 424.004 263.333 267.2 194.196 352 185.5 207 110.5
5 0.466867579 0 10459.3 307.023 4310.14 249.528 11158 253 4303 197
6 0.460607503 0 1062.81 268.847 581.678 231.466 889 180 513 181
7 -0.118948057 0 23004 474.366 8999.72 336.564 25480 392 9934 281
8 1.525603295 0 432.922 264.962 235.787 229.548 361 162.5 186 159.5
9 -0.067695336 0 490.57 203.802 353.051 179.226 414 135 271 97
10 0.3100333 0 263.206 201.014 172.319 164.78 192 137 104 89
11 NULL 0 216.941 210.183 175.388 140.381 145 148 99 67.5
12 -0.464193455 0 266.994 192.826 207.908 151.078 187 140 120.5 78
13 1.00019615 0 1147.27 207.077 398.233 143.161 1009 111.5 295 64
14 -0.090917522 0 228.77 172.556 184.755 142.479 165.5 93 91.5 74
15 0.126912731 0 791.654 260.471 451.956 179.48 679 208 356 103.5
16 -0.241543838 0 388.96 264.339 298.867 235.315 326 215.5 217 152.5
17 -0.18910632 0 1104.26 403.032 749.874 332.558 965 344 646 232
18 0.486778044 0 670.758 251.233 411.251 232.233 556 192 352 170
19 -0.113507272 0 333.74 219.14 233.392 179.627 256 160 161 97
20 0.158798697 0 408.038 289.316 240.554 177.847 329 226.5 159 119.5

Total number of rows: 14720

Table truncated, full table size 992 Kbytes.




Supplementary data files not provided

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