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Status
Public on May 21, 2004
Title
B chip Version2: time 3 hour Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 hour Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
152.122
86.125
174.748
131.984
59
28
74
63.5
2
NULL
0
183.974
153.117
170.048
162.168
114
70.5
85
87.5
3
2.39E+00
0
5217.65
249.65
1570.29
202.997
5099
183.5
1423
111.5
4
-2.33E-01
0
247.224
196.073
238.704
156.095
157.5
103
176.5
79
5
5.12E-01
0
1845.74
176.639
1726.9
190.715
1713
111
1622.5
89.5
6
1.16E-01
0
474.324
194.852
488.76
170.456
391
121
395
93
7
4.25E-01
0
6346.15
239.212
5819.43
259.667
6304
145
5834
182.5
8
2.58E-01
0
267.324
189.177
244.514
187.333
177
96.5
153
105
9
5.09E-01
0
373.139
135.332
355.165
183.661
298
66
264.5
104
10
-7.55E-02
0
186.938
162.235
160.809
125.714
112
75
69
53
11
3.46E-02
0
231.663
165
191.2
131.229
143
74.5
98
44
12
-2.11E-01
0
202.174
167.84
206.193
156.326
116.5
75.5
124.5
71
13
8.25E-01
0
650.923
162.56
507.666
166.883
572
66
429
90.5
14
-1.49E-01
0
214.427
171.563
216.718
160.87
115
87.5
150
86.5
15
4.56E-01
0
798.096
138.174
752.174
173.01
671
58
603
100
16
-1.38E+00
0
244.525
196.46
359.651
181.504
167
133.5
304.5
101.5
17
-4.44E-01
0
852.647
208.259
1262.27
214.827
773.5
160
1163
165.5
18
5.07E-01
0
553.695
182.472
533.454
189.378
486.5
92.5
456
128
19
1.80E-01
0
256.076
156.88
266.75
175.062
170
69
202
102.5
20
-8.45E-02
0
205.353
157.011
216.654
165.156
119
68
140
81
Total number of rows: 14720 Table truncated, full table size 961 Kbytes .Supplementary data files not provided