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Sample GSM22842 Query DataSets for GSM22842
Status Public on May 21, 2004
Title B chip Version2: time 3 hour Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 246.256 187.767 192.071 161.413 196.5 108 107 89.5
2 NULL 0 213.551 202.37 212.57 195.712 149 122.5 135 112.5
3 1.334369026 0 3812.03 270.128 3364.52 295.851 3687.5 222 3246 236
4 0.336626339 0 276.714 191.276 296.169 218.744 230 120 215.5 151.5
5 0.454930954 0 5240.81 230.045 7754.39 272.209 5184 169.5 7673.5 201
6 -0.109702627 0 651.099 174.955 1229.86 199.115 583 97.5 1166.5 134.5
7 0.485182053 0 7228.74 226.09 9926.51 265.951 7203 166.5 9989 220
8 -0.03483873 0 313.965 216.231 337.647 187.388 242 138 247 97
9 -0.311565561 0 412.47 176.99 728.115 166.573 349.5 102 602.5 78
10 NULL 0 202.742 173.938 172.851 148.573 115.5 93 100.5 85.5
11 NULL 0 272.269 175.136 203.436 173.811 173 93.5 119.5 105
12 NULL 0 214.552 180.346 210.843 173.965 136 124 130 107
13 0.978501536 0 822.231 199.017 790.666 201.173 695 127 655 128
14 NULL 0 238.408 204.076 248.876 171.633 169 119.5 179 103
15 -0.318743507 0 636.45 233.927 1181.12 207.646 501 175 1057 133
16 -0.82205125 0 330.442 236.429 592.746 240.897 267.5 164 509 181.5
17 -0.219125456 0 1126.9 286.74 2227.92 323.055 1048 235 2065 246
18 0.164325797 0 581.592 250.496 774.572 223.799 487 171 669 127
19 -0.232980981 0 277.732 160.277 403.962 181.341 213 91.5 316 101.5
20 0.010819508 0 276.846 172.521 350.959 208.188 198.5 111.5 275.5 142.5

Total number of rows: 14720

Table truncated, full table size 992 Kbytes.




Supplementary data files not provided
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