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Sample GSM22841 Query DataSets for GSM22841
Status Public on May 21, 2004
Title B chip Version2: time 3 hour Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1232
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF Reference key to the corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 1 167.799 169.899 194.85 130.106 99 106 131 66
2 NULL 0 177.151 175.103 233.764 207.384 109.5 107.5 180.5 154
3 1.14E+00 0 3038.18 230.784 2830.14 382.905 2945 165 2794 316
4 4.82E-01 0 333.554 178.567 449.317 268.51 246 108.5 385 205
5 3.50E-01 0 7068.68 225.333 10131.5 375.282 7067 175 10369 324
6 -5.81E-02 0 515.488 206.036 932.073 304.803 439 145 795 246
7 -2.58E-01 0 24560.3 541.204 26310.3 833.89 24180 495 26437 789
8 NULL 0 328.701 262.295 589.397 347.783 258 193.5 505 267.5
9 2.34E-01 0 339.126 183.628 409.922 184.392 250 119 328 122
10 NULL 0 186.312 179.272 186.966 158.846 125.5 124.5 134 92
11 NULL 0 156.219 178.681 185.318 156.5 84.5 119.5 116 92.5
12 NULL 0 199.687 143.644 230.86 192.037 131 79 155 110.5
13 9.47E-01 0 595.902 162.191 551.352 174.339 492 102.5 471 105.5
14 NULL 0 189.301 173.87 210.783 211.744 122 106 144 152
15 4.91E-02 0 945.674 161.943 1874.6 262.707 841 91 1646 210
16 -4.71E-01 0 362.335 202.448 782.452 345.579 303 145 719 303
17 -4.63E-01 0 1013.08 287.101 2536.6 544.47 916 267 2447 461
18 2.06E-02 0 533.077 189.242 912.577 271.764 448 141 810 218
19 NULL 0 227.804 146.376 308.555 178.888 150 103 232 91.5
20 NULL 0 241.439 176.36 311.437 234.735 176 112 242 187

Total number of rows: 14720

Table truncated, full table size 948 Kbytes.




Supplementary data files not provided

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