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Status
Public on May 21, 2004
Title
B chip Version2: time 3 hour Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 hour Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1232
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
167.799
169.899
194.85
130.106
99
106
131
66
2
NULL
0
177.151
175.103
233.764
207.384
109.5
107.5
180.5
154
3
1.14E+00
0
3038.18
230.784
2830.14
382.905
2945
165
2794
316
4
4.82E-01
0
333.554
178.567
449.317
268.51
246
108.5
385
205
5
3.50E-01
0
7068.68
225.333
10131.5
375.282
7067
175
10369
324
6
-5.81E-02
0
515.488
206.036
932.073
304.803
439
145
795
246
7
-2.58E-01
0
24560.3
541.204
26310.3
833.89
24180
495
26437
789
8
NULL
0
328.701
262.295
589.397
347.783
258
193.5
505
267.5
9
2.34E-01
0
339.126
183.628
409.922
184.392
250
119
328
122
10
NULL
0
186.312
179.272
186.966
158.846
125.5
124.5
134
92
11
NULL
0
156.219
178.681
185.318
156.5
84.5
119.5
116
92.5
12
NULL
0
199.687
143.644
230.86
192.037
131
79
155
110.5
13
9.47E-01
0
595.902
162.191
551.352
174.339
492
102.5
471
105.5
14
NULL
0
189.301
173.87
210.783
211.744
122
106
144
152
15
4.91E-02
0
945.674
161.943
1874.6
262.707
841
91
1646
210
16
-4.71E-01
0
362.335
202.448
782.452
345.579
303
145
719
303
17
-4.63E-01
0
1013.08
287.101
2536.6
544.47
916
267
2447
461
18
2.06E-02
0
533.077
189.242
912.577
271.764
448
141
810
218
19
NULL
0
227.804
146.376
308.555
178.888
150
103
232
91.5
20
NULL
0
241.439
176.36
311.437
234.735
176
112
242
187
Total number of rows: 14720 Table truncated, full table size 948 Kbytes .Supplementary data files not provided