|
| Status |
Public on May 05, 2017 |
| Title |
HMLE cells engineered with LEX empty vector (Control), replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HMLE cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HMLE
type: Human Mammary epithelial Cell
exogenous manipulation: LEX
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell preparation protocol: HMLE cells were infected with lentiviral vectors expressing miR-199a. Virus containing the empty vector were used as control. Cells were treated and selected with puromycin and the overexpression of miR-199a was confirmed by qPCR.
Total RNA were extracted using RNAeasy mini-prep kit from Qiagen
|
| Label |
Cy5
|
| Label protocol |
The testing RNA samples and universal mouse reference RNA (Agilent) were labeled with CTP-cy5 and CTP-cy3, respectively, Using Agilent Quick Amp Labeling Kit.
|
| |
|
| Channel 2 |
| Source name |
Universal human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell lines: Mix of 10 human cell lines
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell preparation protocol: HMLE cells were infected with lentiviral vectors expressing miR-199a. Virus containing the empty vector were used as control. Cells were treated and selected with puromycin and the overexpression of miR-199a was confirmed by qPCR.
Total RNA were extracted using RNAeasy mini-prep kit from Qiagen
|
| Label |
Cy3
|
| Label protocol |
The testing RNA samples and universal mouse reference RNA (Agilent) were labeled with CTP-cy5 and CTP-cy3, respectively, Using Agilent Quick Amp Labeling Kit.
|
| |
|
| |
| Hybridization protocol |
Each cy5 labeled testing sample was mixed with equal amount cy3-labeled reference sample and fragmented and blocked (Agilent Gene Expression Hybridization Kit), and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
Images were quantified using Agilent Feature Extraction Software (v11.0.1.1).
|
| Description |
See Agilent Cat#740000 for product details
|
| Data processing |
Agilent Feature Extraction Software (v11.0.1.1) was used for background subtraction and linear Lowess normalization.
|
| |
|
| Submission date |
Aug 15, 2016 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Yong Wei |
| E-mail(s) |
yongwei@princeton.edu
|
| Organization name |
Princeton University
|
| Department |
Molecular Biology
|
| Lab |
Kang
|
| Street address |
LTL227 Wathington Road
|
| City |
Plainsboro |
| State/province |
NJ |
| ZIP/Postal code |
08536 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE85629 |
miR-199a regulates human mammary epthelial cell function |
| GSE85808 |
miR-199a-LCOR axis regulation of mammary gland stem cell and breast cancer stem cell transcriptional programs |
|
