|
| Status |
Public on Oct 30, 2016 |
| Title |
miRNAs from PC12 patient |
| Sample type |
RNA |
| |
|
| Source name |
serum miRNA (pancreatic cancer)
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
age: 75
ca19-9: >37
|
| Treatment protocol |
Serum samples were collected in 10-mL BD serum tubes and centrifuged at 4°C for 20 minutes at 3000 rpm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA containing miRNA was extracted from the serum samples using the Serum miRNA purification kit (Genolution, Seoul, Korea) according to the manufacturer’s instructions, and the RNA was resuspended with 12 μL of RNase-free water and stored at -80°C until microarray or qRT-PCR
|
| Label |
biotin
|
| Label protocol |
RNA samples (130 ng) were labelled using the FlashTag™ Biotin RNA Labeling Kit (Genisphere, Hatfield, PA)
|
| |
|
| Hybridization protocol |
labelled RNA was heated to 99°C for 5 minutes and then to 45°C for 5 minutes. RNA-array hybridization was performed with agitation at 60 rotations per minute for 16 hours at 48°C on an Affymetrix® 450 Fluidics Station
|
| Scan protocol |
The chips were washed and stained using a Genechip Fluidics Station 450 (Affymetrix). The chips were then scanned using an Affymetrix GCS 3000 scanner
|
| Description |
serum miRNAs from PC patient
|
| Data processing |
cel files were analysed and rma-normalized by using the Expression Console software
|
| |
|
| Submission date |
Aug 15, 2016 |
| Last update date |
Oct 30, 2016 |
| Contact name |
Jaehoon Lee |
| E-mail(s) |
jae.h.lee@lge.com
|
| Phone |
82-10-9879-9131
|
| Organization name |
LG Electronics
|
| Street address |
38, Baumoe-ro
|
| City |
Seoul |
| ZIP/Postal code |
137-724 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE85589 |
Expression data from pancreatic cancers patients and healthy controls |
|
