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Sample GSM22760 Query DataSets for GSM22760
Status Public on May 21, 2004
Title A chip: time 15 day Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 15 day Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 1.86E-02 0 377.945 188.419 291.856 188.63 292 120.5 217 113
2 -7.22E-01 0 222.889 186.703 409.863 431.339 146 123 338 274
3 3.49E-01 0 1613.37 163.098 1000.93 314.067 1438 88 899 274
4 -9.47E-01 0 194.438 167.307 184.81 113.729 111.5 94 106 29
5 -4.32E-01 0 5630.74 1576.71 3881.28 1225.55 5571 558.5 3835 333.5
6 -4.83E-01 0 530.293 212.658 438.672 165.772 376 142.5 339 65.5
7 -2.82E-01 0 256.986 145.694 217.437 132.62 172.5 70 126 51.5
8 9.80E-01 0 170.832 129.818 126.417 133.521 87 47.5 46 70
9 -8.81E-01 0 2434.66 214.503 2316.67 143.377 1956 105 1815 64
10 -2.29E-01 0 208.359 153.822 188.179 130.679 124 76 112 64
11 -7.40E-01 0 410.479 126.727 483.571 146.823 329 46.5 339 65.5
12 1.95E-01 0 479.02 133.85 318.49 155.526 376 54 244 63
13 -7.37E-01 0 3165.8 156.969 2694.9 122.051 3161 71 2473 52.5
14 -1.32E-01 0 132.622 125.5 122.214 110.252 49 43.5 42 36
15 -2.89E-01 0 140.73 121.706 138.293 115.508 57 48.5 55.5 39
16 -2.09E-01 0 162.545 140.573 158.469 136.013 78 59 76 53
17 3.38E-02 0 912.241 165.371 601.034 121.008 778.5 69 514 53
18 -7.42E-01 0 2110.36 150.615 1957.71 187.074 2019 74 1787 117
19 -4.94E-01 0 215.363 195.048 196.262 142.382 115.5 114 92 74.5
20 2.04E-01 0 225.666 134.473 174.357 129.105 133 46 89 54

Total number of rows: 16928

Table truncated, full table size 1058 Kbytes.




Supplementary data files not provided

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