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Status
Public on May 21, 2004
Title
A chip: time 15 day Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 15 day Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
3.50E-02
0
603.658
298.214
501.538
260.063
502
175
350
169.5
2
-5.26E-01
0
377.75
328.284
412.248
379.692
260
214.5
292
210.5
3
-1.99E-01
0
1239.42
328.35
1105.88
276.122
1100
212.5
934
172
4
5.36E-02
0
376.137
292.946
324.791
242.899
255
166
209.5
149
5
-9.58E-02
0
6188.87
376.778
4877.84
287.556
5778
269
4362
190
6
-3.19E-01
0
705.607
293.85
640.699
256.781
561
210
498
142
7
-3.11E-01
0
325.066
278.189
301.754
214.056
203.5
163
185.5
128.5
8
-2.06E-02
0
294.086
266.304
233.137
220.994
193
167.5
127
117
9
-3.96E-01
0
2103.87
313.168
2018.84
275.858
1833
204
1727
166
10
-1.26E-02
0
341.734
244.783
299.506
243.376
233
150
184
132
11
-5.19E-01
0
549.015
257.85
629.989
261.03
420
131.5
474.5
152.5
12
-1.71E-01
0
505.555
273.594
452.531
243.568
376
155
311
140
13
-3.92E-01
0
3332.44
286.491
3119.02
239.779
3358
187
3008
143
14
-4.76E-01
0
252.461
257.694
232.007
186.857
146.5
149.5
120.5
102.5
15
8.03E-02
0
259.776
234.563
224.695
240.17
157
141
121
121
16
1.51E-01
0
333.378
278.36
276.767
217.761
222.5
138
169.5
124
17
-3.97E-02
0
807.781
261.712
669.548
227.98
716.5
166.5
543
133
18
-3.58E-01
0
1798.27
281.742
1709.67
242.809
1653
177
1442
143
19
-7.92E-02
0
364.467
296.885
306.199
236.127
248
173
186
131
20
1.83E-01
0
351.674
256.612
293.562
243.308
236.5
157
181
144.5
Total number of rows: 16928 Table truncated, full table size 1102 Kbytes .Supplementary data files not provided