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Sample GSM22759 Query DataSets for GSM22759
Status Public on May 21, 2004
Title A chip: time 15 day Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 15 day Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 3.50E-02 0 603.658 298.214 501.538 260.063 502 175 350 169.5
2 -5.26E-01 0 377.75 328.284 412.248 379.692 260 214.5 292 210.5
3 -1.99E-01 0 1239.42 328.35 1105.88 276.122 1100 212.5 934 172
4 5.36E-02 0 376.137 292.946 324.791 242.899 255 166 209.5 149
5 -9.58E-02 0 6188.87 376.778 4877.84 287.556 5778 269 4362 190
6 -3.19E-01 0 705.607 293.85 640.699 256.781 561 210 498 142
7 -3.11E-01 0 325.066 278.189 301.754 214.056 203.5 163 185.5 128.5
8 -2.06E-02 0 294.086 266.304 233.137 220.994 193 167.5 127 117
9 -3.96E-01 0 2103.87 313.168 2018.84 275.858 1833 204 1727 166
10 -1.26E-02 0 341.734 244.783 299.506 243.376 233 150 184 132
11 -5.19E-01 0 549.015 257.85 629.989 261.03 420 131.5 474.5 152.5
12 -1.71E-01 0 505.555 273.594 452.531 243.568 376 155 311 140
13 -3.92E-01 0 3332.44 286.491 3119.02 239.779 3358 187 3008 143
14 -4.76E-01 0 252.461 257.694 232.007 186.857 146.5 149.5 120.5 102.5
15 8.03E-02 0 259.776 234.563 224.695 240.17 157 141 121 121
16 1.51E-01 0 333.378 278.36 276.767 217.761 222.5 138 169.5 124
17 -3.97E-02 0 807.781 261.712 669.548 227.98 716.5 166.5 543 133
18 -3.58E-01 0 1798.27 281.742 1709.67 242.809 1653 177 1442 143
19 -7.92E-02 0 364.467 296.885 306.199 236.127 248 173 186 131
20 1.83E-01 0 351.674 256.612 293.562 243.308 236.5 157 181 144.5

Total number of rows: 16928

Table truncated, full table size 1102 Kbytes.




Supplementary data files not provided

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