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Sample GSM22758 Query DataSets for GSM22758
Status Public on May 21, 2004
Title A chip: time 15 day Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 15 day Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 2.86E-01 0 397.224 251.166 318.395 255.916 259.5 148 180.5 139
2 -5.16E-01 0 288.741 276.028 333.403 319.274 163 169 209 183
3 7.49E-02 0 1176.88 253.887 1008.58 281.785 1034 153 825 177.5
4 -4.06E-01 0 300.755 297.034 273.815 223.024 185 181 158 133.5
5 -1.80E-01 0 3979.08 314.371 3521.28 256.78 3681 206 3065 153
6 -4.05E-01 0 528.936 303.144 525.204 233.501 413 175 377 131
7 -4.43E-01 0 272.39 248.087 259.88 196.279 170.5 133 140 97
8 2.39E-01 0 270.332 212.15 199.83 161.85 150 113.5 99 84
9 -2.99E-01 0 1653.48 283.821 1618.5 231.226 1412 173.5 1311 124.5
10 3.29E-01 0 306.49 224.066 237.736 196.05 186 119.5 123 109
11 -5.22E-01 0 401.057 234.439 454.763 205.343 257 125 320 120.5
12 3.90E-01 0 472.39 261.738 315.016 210.878 344 153 181 108
13 -8.04E-01 0 2356.7 305.875 3111.59 233.38 2236 189.5 2906 117
14 NULL 0 260.817 267.253 210.156 190.705 152.5 152 108 103.5
15 -9.98E-02 0 256.431 249.471 207.527 187.817 142 157 120 106
16 -5.72E-01 0 270.087 259.145 252.795 213.107 154 150 137 97
17 6.18E-02 0 683.348 266.242 593.446 289.37 572 138 444 146
18 -7.43E-01 0 1283.53 265.635 1679.14 217.919 1171 163 1497 116
19 -5.78E-01 0 311.03 269.623 310.694 211.623 182.5 168.5 190 119
20 2.29E-01 0 300.296 242.632 261.2 253.095 183.5 136 152 141

Total number of rows: 16928

Table truncated, full table size 1101 Kbytes.




Supplementary data files not provided

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