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Status
Public on May 21, 2004
Title
A chip: time 15 day Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 15 day Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
2.86E-01
0
397.224
251.166
318.395
255.916
259.5
148
180.5
139
2
-5.16E-01
0
288.741
276.028
333.403
319.274
163
169
209
183
3
7.49E-02
0
1176.88
253.887
1008.58
281.785
1034
153
825
177.5
4
-4.06E-01
0
300.755
297.034
273.815
223.024
185
181
158
133.5
5
-1.80E-01
0
3979.08
314.371
3521.28
256.78
3681
206
3065
153
6
-4.05E-01
0
528.936
303.144
525.204
233.501
413
175
377
131
7
-4.43E-01
0
272.39
248.087
259.88
196.279
170.5
133
140
97
8
2.39E-01
0
270.332
212.15
199.83
161.85
150
113.5
99
84
9
-2.99E-01
0
1653.48
283.821
1618.5
231.226
1412
173.5
1311
124.5
10
3.29E-01
0
306.49
224.066
237.736
196.05
186
119.5
123
109
11
-5.22E-01
0
401.057
234.439
454.763
205.343
257
125
320
120.5
12
3.90E-01
0
472.39
261.738
315.016
210.878
344
153
181
108
13
-8.04E-01
0
2356.7
305.875
3111.59
233.38
2236
189.5
2906
117
14
NULL
0
260.817
267.253
210.156
190.705
152.5
152
108
103.5
15
-9.98E-02
0
256.431
249.471
207.527
187.817
142
157
120
106
16
-5.72E-01
0
270.087
259.145
252.795
213.107
154
150
137
97
17
6.18E-02
0
683.348
266.242
593.446
289.37
572
138
444
146
18
-7.43E-01
0
1283.53
265.635
1679.14
217.919
1171
163
1497
116
19
-5.78E-01
0
311.03
269.623
310.694
211.623
182.5
168.5
190
119
20
2.29E-01
0
300.296
242.632
261.2
253.095
183.5
136
152
141
Total number of rows: 16928 Table truncated, full table size 1101 Kbytes .Supplementary data files not provided