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Status
Public on May 21, 2004
Title
A chip: time 24 hour Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
1.53E-01
0
337.34
265.058
485.909
449.825
275
176.5
407
360.5
2
-1.55E+00
0
819.226
745.055
1866.6
1687.96
723
532
1513
1090
3
5.27E-01
0
4405.24
294.5
2573.27
610.626
4178
236
2200
516
4
NULL
0
252.148
256.663
400.894
380.74
178
185
340
339
5
-5.62E-01
0
4386.24
234.421
4407.54
298.042
4235
158
4262
221
6
-6.89E-01
0
547.707
207.204
806.186
238.995
470
116
671
160
7
-2.41E-01
0
276.36
174.838
328.096
206.324
184
86
250.5
130.5
8
3.66E-01
0
197.068
179.988
200.528
209.115
124
106
123
142
9
-9.00E-01
0
1665.96
215.067
2330.84
201.949
1399
139
1823
148
10
-2.58E-01
0
213.315
168.418
238.128
190.762
131
98.5
161
116.5
11
-3.92E-01
0
365.791
182.404
414.328
204.653
278
116
305
124
12
7.16E-01
0
1242.57
185.136
739.448
186.572
802
116
441
116
13
-8.98E-01
0
2002.22
203.621
2749.12
214.807
1585
135
1904
165
14
-5.89E-01
0
182.621
191.047
171.406
153.812
103
120
94
93
15
3.16E-01
0
206
161.553
183.306
187.043
117
80
108.5
91.5
16
-1.99E-01
0
219.604
148.004
229.317
172.136
135.5
85.5
140.5
96
17
1.15E-01
0
641.727
177.262
611.035
209.119
504
111
462
155.5
18
-7.63E-01
0
983.219
179.819
1448.25
220.858
812
108
1150
163
19
9.17E-01
0
205.022
154.478
187.473
195.662
118
67
101.5
122
20
7.48E-01
0
326.399
192.275
253.71
231.466
244
109.5
167
140
Total number of rows: 16928 Table truncated, full table size 1091 Kbytes .Supplementary data files not provided