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Sample GSM22743 Query DataSets for GSM22743
Status Public on May 21, 2004
Title A chip: time 24 hour Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 1.53E-01 0 337.34 265.058 485.909 449.825 275 176.5 407 360.5
2 -1.55E+00 0 819.226 745.055 1866.6 1687.96 723 532 1513 1090
3 5.27E-01 0 4405.24 294.5 2573.27 610.626 4178 236 2200 516
4 NULL 0 252.148 256.663 400.894 380.74 178 185 340 339
5 -5.62E-01 0 4386.24 234.421 4407.54 298.042 4235 158 4262 221
6 -6.89E-01 0 547.707 207.204 806.186 238.995 470 116 671 160
7 -2.41E-01 0 276.36 174.838 328.096 206.324 184 86 250.5 130.5
8 3.66E-01 0 197.068 179.988 200.528 209.115 124 106 123 142
9 -9.00E-01 0 1665.96 215.067 2330.84 201.949 1399 139 1823 148
10 -2.58E-01 0 213.315 168.418 238.128 190.762 131 98.5 161 116.5
11 -3.92E-01 0 365.791 182.404 414.328 204.653 278 116 305 124
12 7.16E-01 0 1242.57 185.136 739.448 186.572 802 116 441 116
13 -8.98E-01 0 2002.22 203.621 2749.12 214.807 1585 135 1904 165
14 -5.89E-01 0 182.621 191.047 171.406 153.812 103 120 94 93
15 3.16E-01 0 206 161.553 183.306 187.043 117 80 108.5 91.5
16 -1.99E-01 0 219.604 148.004 229.317 172.136 135.5 85.5 140.5 96
17 1.15E-01 0 641.727 177.262 611.035 209.119 504 111 462 155.5
18 -7.63E-01 0 983.219 179.819 1448.25 220.858 812 108 1150 163
19 9.17E-01 0 205.022 154.478 187.473 195.662 118 67 101.5 122
20 7.48E-01 0 326.399 192.275 253.71 231.466 244 109.5 167 140

Total number of rows: 16928

Table truncated, full table size 1091 Kbytes.




Supplementary data files not provided

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