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Status
Public on May 21, 2004
Title
A chip: time 24 hour Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-4.47E-02
0
511.821
293.766
442.479
346.853
420
237.5
394
246.5
2
-1.74E-01
0
618.685
463.841
757.005
706.511
547
352.5
687
549
3
4.06E-01
0
9123.24
432.244
4120.05
585.569
7478
363
3631
514
4
-1.05E+00
0
388.152
316.053
428.827
287.316
326
280
362.5
242.5
5
-4.12E-01
0
9035.75
320.873
6641.65
378.88
8635
243
6087
304
6
-3.91E-01
0
997.246
255.519
863.208
280.171
783
187.5
664
197.5
7
-4.05E-01
0
465.277
245.641
436.798
244.481
414
166
364
166
8
-1.32E-02
0
230.873
209.378
259.012
218.611
167.5
104
183
146.5
9
-5.22E-01
0
3354.59
241.463
2837.54
261.541
2437
185.5
2136
198
10
5.45E-02
0
265.849
245.256
256.825
231.065
191
172
184
175
11
-3.20E-01
0
614.772
230.906
502.095
202.701
496
157
398
130.5
12
4.20E-01
0
4112.63
250.497
1842.82
208.849
3523
159
1471
129.5
13
-5.98E-01
0
3835.02
254.919
3370.98
247.267
3468
175
2847
198
14
-6.77E-03
0
220.2
190.23
215.454
208.352
159
121.5
134
126
15
1.04E-01
0
251.039
213.914
222.637
188.014
176.5
133
136
125
16
3.41E-01
0
307.704
222.346
247.07
233.805
230
166
171
149
17
2.18E-01
0
939.607
250.745
539.41
198.769
718
188
426
135
18
-4.04E-01
0
2048.27
251.326
1676.85
232.211
1485
177
1275
157.5
19
-7.20E-01
0
208.652
206.087
208.195
179.553
149
137
128
100.5
20
-6.81E-01
0
370.862
230.304
328.556
182.53
293
170
225
101.5
Total number of rows: 16928 Table truncated, full table size 1096 Kbytes .Supplementary data files not provided