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Sample GSM22741 Query DataSets for GSM22741
Status Public on May 21, 2004
Title A chip: time 24 hour Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -4.47E-02 0 511.821 293.766 442.479 346.853 420 237.5 394 246.5
2 -1.74E-01 0 618.685 463.841 757.005 706.511 547 352.5 687 549
3 4.06E-01 0 9123.24 432.244 4120.05 585.569 7478 363 3631 514
4 -1.05E+00 0 388.152 316.053 428.827 287.316 326 280 362.5 242.5
5 -4.12E-01 0 9035.75 320.873 6641.65 378.88 8635 243 6087 304
6 -3.91E-01 0 997.246 255.519 863.208 280.171 783 187.5 664 197.5
7 -4.05E-01 0 465.277 245.641 436.798 244.481 414 166 364 166
8 -1.32E-02 0 230.873 209.378 259.012 218.611 167.5 104 183 146.5
9 -5.22E-01 0 3354.59 241.463 2837.54 261.541 2437 185.5 2136 198
10 5.45E-02 0 265.849 245.256 256.825 231.065 191 172 184 175
11 -3.20E-01 0 614.772 230.906 502.095 202.701 496 157 398 130.5
12 4.20E-01 0 4112.63 250.497 1842.82 208.849 3523 159 1471 129.5
13 -5.98E-01 0 3835.02 254.919 3370.98 247.267 3468 175 2847 198
14 -6.77E-03 0 220.2 190.23 215.454 208.352 159 121.5 134 126
15 1.04E-01 0 251.039 213.914 222.637 188.014 176.5 133 136 125
16 3.41E-01 0 307.704 222.346 247.07 233.805 230 166 171 149
17 2.18E-01 0 939.607 250.745 539.41 198.769 718 188 426 135
18 -4.04E-01 0 2048.27 251.326 1676.85 232.211 1485 177 1275 157.5
19 -7.20E-01 0 208.652 206.087 208.195 179.553 149 137 128 100.5
20 -6.81E-01 0 370.862 230.304 328.556 182.53 293 170 225 101.5

Total number of rows: 16928

Table truncated, full table size 1096 Kbytes.




Supplementary data files not provided

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