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Status
Public on May 21, 2004
Title
A chip: time 8 hour Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-4.39E-01
0
97.806
67.054
118.677
66.7446
21
13
37
10
2
3.95E-01
0
112
65.5296
90.3095
76.0987
35.5
10
21
19
3
-1.43E-01
0
634.145
93.6071
839.356
100.27
514
27.5
656
29.5
4
-2.16E-01
0
114.95
79.3224
142.143
87.8191
35
10
50
19
5
7.01E-01
0
3501.92
129.566
2032.95
130.427
3225.5
36
1708.5
48
6
-1.16E-01
0
146.133
82.2857
162.693
72.1865
62
15
73
15
7
-1.01E-01
0
134.887
83.0897
143.803
67.2379
52.5
13
58
9
8
NULL
0
74.1799
63.971
77.9422
83.6377
13
10
17
14
9
-5.77E-01
0
867.099
168.809
1381.56
148.511
728
65
1110
59.5
10
2.44E-01
0
110.847
69.4783
94.7923
89.9348
35
12
20
18
11
-6.79E-01
0
228.69
83.7118
418.294
87.4653
134
15.5
356
18.5
12
NULL
0
131.614
76.7619
140.507
67.3373
31
13.5
45
11
13
-4.99E-01
0
160.157
75.3547
241.763
79.141
64
14.5
111
22.5
14
-3.53E-01
0
72.3024
58.1236
84.5574
71.5891
11
10
20
13
15
NULL
0
92.144
69.5388
105.541
65.6408
19.5
14.5
27
11
16
-1.18E-01
0
98.3294
88.7725
90.9911
69.3704
20
20
19
13
17
5.08E-02
0
337.856
80.125
393.929
72.5799
249.5
10.5
276.5
14
18
-5.61E-01
0
457.08
73.0683
832.291
75.3849
369.5
12.5
639
11
19
-3.91E-02
0
85.5974
61.2706
83.4845
75.7765
17
11
18
14
20
1.14E-02
0
89.496
77.6781
81.373
73.3185
17.5
12
18
11.5
Total number of rows: 16928 Table truncated, full table size 1035 Kbytes .Supplementary data files not provided