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Status
Public on May 21, 2004
Title
A chip: time 8 hour Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-4.22E-01
0
94.3196
59.8157
113.765
59.898
19
8
36
8
2
4.49E-01
0
96.7088
69.0133
61.606
47.9967
27
12
11
7
3
5.24E-01
0
391.557
57.6511
305.202
77.3525
287
7.5
199
17.5
4
-3.16E-01
0
88.372
79.3185
97.1017
86.5185
17
13.5
20
13
5
6.37E-01
0
1137.81
108.316
687.636
90.398
958
25.5
592
28
6
NULL
0
98.2262
75.1404
127.188
79.0308
22.5
13.5
47
14.5
7
-6.53E-01
0
88.0482
80.5764
108.606
64.2083
19
17
29.5
9
8
1.82E-01
0
87.8761
49.4909
71.1147
58.6636
14
7.5
9
8
9
-1.07E+00
0
476.306
82.0479
948.665
80.3801
358.5
15.5
678
17
10
NULL
0
87.7798
69.3421
91.6687
71.2434
14
11
20.5
11
11
-7.06E-01
0
129.878
78.0809
203.811
81.9515
39
10
92
17
12
5.48E-01
0
175.795
82.2103
113.849
59.1111
54
15
28
10
13
-4.85E-01
0
145.73
64.5434
189.454
64.1918
58
12
70
10
14
1.73E-01
0
71.3234
79.5514
74.7092
72.4074
14
10
9
20
15
2.32E-02
0
87.8424
51.354
78.212
53.1204
19
8
9.5
7
16
2.10E-01
0
96.0593
62.4021
81.6884
73.1376
18
11
12
15
17
1.62E-01
0
200.144
69.3309
180.03
76.4388
108
8.5
96
14
18
-9.85E-02
0
266.397
64.2093
294.839
75.4535
151
12
188.5
10
19
4.02E-01
0
84.4176
56.7831
66.7002
72.7757
11
10
11
15
20
-2.14E-01
0
97.4817
63.1076
100.748
64.9792
23.5
8
30
7
Total number of rows: 16928 Table truncated, full table size 1023 Kbytes .Supplementary data files not provided