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Sample GSM22724 Query DataSets for GSM22724
Status Public on May 21, 2004
Title A chip: time 1 hour Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) fangy@ohsu.edu
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 1.18E-01 0 577.069 279.783 675.541 444.603 484 186 588 365.5
2 -2.94E-01 0 615.035 572.121 834.676 886.786 526 439 787 657
3 1.97E-01 0 13843.5 401.179 6480.98 427.764 15019 303 6469 361
4 -3.03E-01 0 258.682 209.059 348.647 286.986 174 131.5 286 216
5 -8.39E-01 0 6362.81 256.079 5828.43 333.419 5729 175 5313 281
6 -1.19E-01 0 949.162 175.882 925.998 270.773 861 98.5 755 200
7 -4.61E-01 0 304.579 149.733 418.679 225.681 231 61 317 136
8 -1.28E-01 0 341.536 144.554 484.298 263.428 251 58 350 218.5
9 -1.50E+00 0 1437.26 154.5 2561.65 265.425 1322 69 2241 208
10 -3.83E-02 0 175.961 141.219 244.926 234.508 91.5 64 170.5 147
11 -4.01E-01 0 309.09 146.643 411.008 245.023 244.5 66.5 336.5 140
12 -1.85E-01 0 121.933 132.584 205.06 224.722 52 62 122 154
13 -1.12E+00 0 3453.88 174.409 4029.31 274.208 3574 114 4131 191.5
14 1.38E-01 0 141.838 153.101 218.517 227.436 67 81.5 144 176
15 -5.98E-01 0 184.902 155.628 245.543 187.416 104 79 168 111.5
16 2.09E-01 0 203.645 131.51 270.342 218.545 122 47 186 153
17 -1.57E-02 0 500.281 157.702 513.208 233.116 447 80 448 153
18 -7.18E-01 0 1246.03 155.201 1479.21 221.706 1089.5 77 1166.5 147.5
19 -2.16E-01 0 160.364 128.899 232.253 202.835 76 54.5 164 128.5
20 2.88E-01 0 289.149 180.196 303.559 230.485 201 94 221 168

Total number of rows: 16928

Table truncated, full table size 1095 Kbytes.




Supplementary data files not provided

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