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Status
Public on May 21, 2004
Title
A chip: time 1 hour Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
fangy@ohsu.edu
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
1.18E-01
0
577.069
279.783
675.541
444.603
484
186
588
365.5
2
-2.94E-01
0
615.035
572.121
834.676
886.786
526
439
787
657
3
1.97E-01
0
13843.5
401.179
6480.98
427.764
15019
303
6469
361
4
-3.03E-01
0
258.682
209.059
348.647
286.986
174
131.5
286
216
5
-8.39E-01
0
6362.81
256.079
5828.43
333.419
5729
175
5313
281
6
-1.19E-01
0
949.162
175.882
925.998
270.773
861
98.5
755
200
7
-4.61E-01
0
304.579
149.733
418.679
225.681
231
61
317
136
8
-1.28E-01
0
341.536
144.554
484.298
263.428
251
58
350
218.5
9
-1.50E+00
0
1437.26
154.5
2561.65
265.425
1322
69
2241
208
10
-3.83E-02
0
175.961
141.219
244.926
234.508
91.5
64
170.5
147
11
-4.01E-01
0
309.09
146.643
411.008
245.023
244.5
66.5
336.5
140
12
-1.85E-01
0
121.933
132.584
205.06
224.722
52
62
122
154
13
-1.12E+00
0
3453.88
174.409
4029.31
274.208
3574
114
4131
191.5
14
1.38E-01
0
141.838
153.101
218.517
227.436
67
81.5
144
176
15
-5.98E-01
0
184.902
155.628
245.543
187.416
104
79
168
111.5
16
2.09E-01
0
203.645
131.51
270.342
218.545
122
47
186
153
17
-1.57E-02
0
500.281
157.702
513.208
233.116
447
80
448
153
18
-7.18E-01
0
1246.03
155.201
1479.21
221.706
1089.5
77
1166.5
147.5
19
-2.16E-01
0
160.364
128.899
232.253
202.835
76
54.5
164
128.5
20
2.88E-01
0
289.149
180.196
303.559
230.485
201
94
221
168
Total number of rows: 16928 Table truncated, full table size 1095 Kbytes .Supplementary data files not provided