|
| Status |
Public on Aug 09, 2016 |
| Title |
Aspergillus nidulans (sirAdelta) 48 h |
| Sample type |
RNA |
| |
|
| Source name |
Aspergillus nidulans (sirAdelta) grown for 48 h
|
| Organism |
Aspergillus nidulans |
| Characteristics |
strain: SirAd (biA1; argB2; sirA::argB)
growth time: 48 h
|
| Treatment protocol |
Mycelia were harvested by filtration and washed by ddH2O.
|
| Growth protocol |
Conidia were incubated for 24, 48, and 72 h at 30oC and 120 rpm in 500-ml Erlenmeyer flasks containing 200 ml of MM medium (1% glucose, 0.6% NaNO3, 10 mM KH2PO4, 7 mM KCl, 2 mM MgSO4, 0.25 mg l-1 biotin and 0.2% Hutner's trace metals (v/v)) (Barratt et al. 1965).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mycelia were immediately frozen in liquid nitrogen and powdered to prepare total RNA using the RNeasy plant mini kit (Qiagen, Venlo, Netherlands).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 250 ng total RNA using GeneChip 3' IVT Express Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip custom Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Genechips were scanned using a GeneChip Scanner 3000 (Affymetrix).
|
| Data processing |
The data were analyzed with GCOS program (version 1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
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| |
|
| Submission date |
Aug 08, 2016 |
| Last update date |
Aug 09, 2016 |
| Contact name |
Eriko Itoh |
| Organization name |
university of tsukuba
|
| Street address |
1-1-1 tennodai
|
| City |
tsukuba |
| ZIP/Postal code |
305-8572 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10798 |
| Series (1) |
| GSE85319 |
Expression data from Aspergillus nidulans FGSCA26 and SirAdelta grown for 24,48, and 72h |
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