|
| Status |
Public on Jul 22, 2016 |
| Title |
NT IFN lambda |
| Sample type |
RNA |
| |
|
| Source name |
Control Huh-7.5 cells treated with interferon lambda
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Huh-7.5
treatment: treated with interferon lambda
genotype/variation: control
|
| Treatment protocol |
Control and STAT1 knockout Huh-7.5 hepatoma cells were left untreated or treated with 1000 U/ml of human interferon alpha 2a or interferon lambda 1 (PBL) for 24 h.
|
| Growth protocol |
Huh-7.5 cells were cultured in DMEM containig 10% FBS and NEAA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy mini kit (Qiagen) according to the manufacture's instruction.
|
| Label |
biotin
|
| Label protocol |
Labeled, Sense-strand cDNA were generated from 200 ng of total RNA using a CHIP WT plus kit (Affymetrix) according to the manufacture's instruction.
|
| |
|
| Hybridization protocol |
Labeled cDNA were hybridized for 16 h at 45C on GeneChip HuGene 2.0 ST Array (Affymetrix). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Genechips were scanned using GCS3000-7G Scanner.
|
| Description |
Gene expression data from Huh-7.5 cells
|
| Data processing |
Data were analyzed with Affymetrix GCS3000 system.
|
| |
|
| Submission date |
Jul 21, 2016 |
| Last update date |
Jul 22, 2016 |
| Contact name |
Shota Yamauchi |
| Organization name |
University of Fukui
|
| Department |
Faculty of Medical Sciences
|
| Lab |
Genome Science and Microbiology
|
| Street address |
23-3 Matsuokashimoaizuki
|
| City |
Eiheiji |
| State/province |
Fukui |
| ZIP/Postal code |
910-1193 |
| Country |
Japan |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE84693 |
Interferon-stimulated gene expression in STAT1 knockout cells |
|
