|
| Status |
Public on Jul 15, 2016 |
| Title |
Low1, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Low concentration group, 30μM
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2
tissue: human hepatocellular carcinoma
|
| Treatment protocol |
HepG2 cells were seeded in 10 cm dishes and cultured as usual until they reached 80% confluence. The cells were treated with H2O2 (0, 30 and 600 μM) for 24 h and each concentration was repeated in 3 dishes.
|
| Growth protocol |
HepG2 cells were maintained as a monolayer culture in DMEM supplemented with 10% fetal bovine serum, 100 IU/ml penicillin and 100 μg/ml streptomycin. HepG2 cells were cultured in a humidified incubator with 5% CO2 at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Per RNA sample, 1 ug were used as input into the procedure as recommended by protocol (Genisphere FlashTagTM Biotin RNA Labeling for Affymetrix GeneChip miRNA arrays).
|
| |
|
| Hybridization protocol |
Labeled miRNA was hybridized to the array for 16 hours at 48 “C and 60 rpm as described in the protocol.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G
|
| Description |
miRNAs expression data from HepG2 cells treated with 30μM H2O2 for 24 hours
|
| Data processing |
The data were analyzed with Robust Multi-chip Average (RMA) algorithm as normalization method.
|
| |
|
| Submission date |
Jul 14, 2016 |
| Last update date |
Jul 15, 2016 |
| Contact name |
Yuan LUO |
| E-mail(s) |
lynavy@qq.com
|
| Organization name |
Air Force General Hospital, PLA, P. R. China
|
| Lab |
Aviation Medicine Research Laboratory
|
| Street address |
No. 30 Fucheng Road, Haidian District
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100142 |
| Country |
China |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE84406 |
miRNAs array analysis of HepG2 cells under oxidative stress |
|
