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Sample GSM2212053 Query DataSets for GSM2212053
Status Public on Jul 15, 2016
Title H3K27ac (ChIP-exo)
Sample type SRA
Source name t(8;21)-containing acute myeloid leukemia cell line
Organism Homo sapiens
Characteristics cell line: Kasumi-1 cells
treatment: none
Treatment protocol none
Growth protocol RPMI 1640 supplemented with 10% FetalPlex and 2 mM L-Glutamine
Extracted molecule genomic DNA
Extraction protocol Cells (50 million) were fixed with 1% formaldehyde for 10 minutes at room temperature and reaction was quenched with 125 mM Glycine. Fragmented chromatin was immunoprecipitated with H3K27ac antibody.
ChIP-exo libraries were prepared according to the published method:
Library strategy OTHER
Library source genomic
Library selection other
Instrument model Illumina HiSeq 2000
Data processing Library strategy: ChIP-exo
Base-calling, filtering, and demultiplexing were performed using Illumina CASAVA 1.8.2 with default settings.
Raw reads were processed to trim adaptor and low quality sequences using Trimmomatic (version 0.32) with parameters: -phred33 input.txt.gz output.txt.gz ILLUMINACLIP:adaptor.txt:2:30:7 TRAILING:15.
For PRO-seq, reverse complements of the trimmed reads were generated using “fastx_reverse_complement” (version 0.0.13) with default settings, and the reverse complement sequences were aligned to the human genome hg19 using bowtie2 (version 2.2.4) with default settings. For ChIP-exo, pre-processed reads were aligned to hg19 using bowtie2 with default settings. For RNA-seq, pre-processed reads were aligned to hg19 using TopHat (version 2.0.10) with default settings.
H3K27ac peak calling was performed using MACS2 (version 2.0.10) with parameters: MACS2 callpeak -t H3K27ac.bam -c Input.bam --nomodel -f BAM -g hs -n -B.
Genome_build: hg19
Supplementary_files_format_and_content: Genome coverage was normalized and calculated for each strand separately and reported in bedGraph format. The ChIP-exo peak file reports genome coordinates, peak length, read counts, and enrichment significance for each called H3K27ac peak. RNA-seq count files contain gene RefSeq IDs and their normalized read counts in reads per kilobase of transcript per million mapped reads (RPKM).
Submission date Jun 23, 2016
Last update date May 15, 2019
Contact name Scott W Hiebert
Phone 615-936-3582
Organization name Vanderbilt University
Department Biochemistry
Lab Hiebert Lab
Street address 2220 Pierce Ave
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
Platform ID GPL11154
Series (1)
GSE83660 High Resolution Mapping of RNA Polymerases Identifies Mechanisms of Sensitivity and Resistance to BET Inhibitors in t(8;21) AML
BioSample SAMN05290369
SRA SRX1873254

Supplementary file Size Download File type/resource
GSM2212053_H3K27ac-ChIPexo_peaks.txt.gz 1.6 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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