|
| Status |
Public on Aug 16, 2007 |
| Title |
Breast Cancer Cell-line BT549 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
genomic DNA from breast cell-lineBT549
|
| Organism |
Homo sapiens |
| Characteristics |
Breast Cancer Cell Line (Female)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Proteinase K/SDS (according to Maniatis)
|
| Label |
CY3
|
| Label protocol |
DNA labelling protocol available via the VUMC MACF website. Also described in van den Ijssel et al, NAR 2005 Dec 16; 33(22):e192
|
| |
|
| Channel 2 |
| Source name |
genomic DNA from male reference DNA
|
| Organism |
Homo sapiens |
| Characteristics |
Pooled DNA from the blood of 10 normal male individuals
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Proteinase K/SDS (according to Maniatis)
|
| Label |
CY5
|
| Label protocol |
DNA labelling protocol available via the VUMC MACF website. Also described in van den Ijssel et al, NAR 2005 Dec 16; 33(22):e192
|
| |
|
| |
| Hybridization protocol |
Hybridisation protocol available via the VUMC MACF website. Also described in van den Ijssel et al, NAR 2005 Dec 16; 33(22):e192
|
| Scan protocol |
Microarray Scanner G2505B (Agilent Technologies) default settings
|
| Description |
No additional information
|
| Data processing |
Spot analysis and QC was fully automated using BlueFuse version 3.1 (BlueGnome, Cambrdige UK); mode-normalisation.
|
| |
|
| Submission date |
Aug 13, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Andrew E Teschendorff |
| E-mail(s) |
a.teschendorff@ucl.ac.uk
|
| Phone |
+44 02076790727
|
| Organization name |
University College London
|
| Department |
UCL Cancer Institute/Institute for Women's Health
|
| Street address |
72 Huntley Street
|
| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL5737 |
| Series (1) |
| GSE8757 |
High resolution array-CGH and expression profiling identifies a novel genomic subtype of ER negative breast cancer |
|
