|
| Status |
Public on Feb 29, 2008 |
| Title |
LNCaP_AndrogenDeprived_5month_2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP_AndrogenDeprived_5month_2, LNCaP cells collected at androgen deprivation for 5 months.
|
| Organism |
Homo sapiens |
| Characteristics |
Cultured prostate cancer cells deprived of androgen for 5 months;
|
| Treatment protocol |
Under androgen deprivation treatment for 5 months.
|
| Growth protocol |
LNCaP cells (obtained from ATCC) were cultured in growth media (RPMI plus 10% FBS, 1% P/S) and used as control, untreated cells. Treated cells were initially plated at 2x106 and deprived at approximately 80% confluence.Deprived cells were passaged once following initial deprivation and not split until after month 10, when they were passaged approximately once per 10 days and plated at 1x106 cells in 75cm2 flasks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNA-Bee as per manufacture's instructions and further cleaned up with Qiagen Rneasy Mini kit following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, 2002, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from non-treated LNCaP cells at time point 0.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Aug 06, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Beth R. Pflug |
| E-mail(s) |
pflugbr@upmc.edu
|
| Phone |
412-623-3932
|
| Organization name |
University of Pittsburgh
|
| Department |
Urology
|
| Lab |
G-43
|
| Street address |
5200 Centre Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15232 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8702 |
Longitudinal Analysis of Progression to Androgen Independence |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
