|
| Status |
Public on Apr 29, 2016 |
| Title |
RPMI8226 hypoxia_miRNA |
| Sample type |
RNA |
| |
|
| Source name |
RPMI8226 hypoxia
|
| Organism |
Homo sapiens |
| Characteristics |
condition: hypoxia
cell line: RPMI8226
cell type: myeloma
|
| Treatment protocol |
normoxia: 21%O2, hypoxia: 1%O2.
|
| Growth protocol |
RPMI1640 plus 10% FCS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol.
|
| Label |
Cy3
|
| Label protocol |
As materials and methods for miRNA microarray protocol version 2.4 (Agilent).
|
| |
|
| Hybridization protocol |
As materials and methods for miRNAmicroarray protocol version 2.4 (Agilent).
|
| Scan protocol |
Agilent G2600A SureScan Microarray Scanner System.
|
| Data processing |
GeneSpring (Agilent), computed normalized signal intensity with 90%tile normalization.
|
| |
|
| Submission date |
Apr 28, 2016 |
| Last update date |
Apr 29, 2016 |
| Contact name |
Sho Ikeda |
| E-mail(s) |
sikeda@med.akita-u.ac.jp
|
| Organization name |
Akita univ.
|
| Department |
Hematology
|
| Lab |
Tagawa labo
|
| Street address |
Hondo 1-1-1
|
| City |
Akita |
| ZIP/Postal code |
010-8543 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18402 |
| Series (1) |
| GSE80769 |
miRNA expression of myeloma cell lines under hypoxia |
|
