|
Status |
Public on Apr 13, 2017 |
Title |
E0040_FASD |
Sample type |
genomic |
|
|
Source name |
E0040
|
Organism |
Homo sapiens |
Characteristics |
cell type: Buccal epithelial cells date run: Apr-12 Sex: Female disease status: FASD disease subgroup: ARND age: 16 self-reported ethnicity: first nations sentrix id: 9247377219 sentrix position: R06C02 sentrix row: R06
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated using the Isohelix DNA isolation kit and bisulfite converted with the Zymo EZ DNA Methylation Kit, as per manufacturer’s instructions.
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridized to Illumina Infinium HumanMethylation450k Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged with BeadArray Reader using standard recommended Illumina scanner setting
|
Data processing |
GenomeStudio software V2011.1 with GenomeStudio Methylation Analysis Module Version 1.9.1000 (background-subtraction, colour-correction). The file unmethylated_and_methylated_signal.txt contains the raw unmethylated (A) and methylated (B) signal intensities.
|
|
|
Submission date |
Apr 13, 2016 |
Last update date |
Apr 13, 2017 |
Contact name |
Michael S. Kobor |
Organization name |
Centre for Molecular Medicine and Therapeutics / University of British Columbia
|
Department |
Medical Genetics
|
Lab |
Kobor
|
Street address |
950 West 28th Avenue
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V5Z 4H4 |
Country |
Canada |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE80261 |
DNA methylation signature of human fetal alcohol spectrum disorder |
|