|
| Status |
Public on Jul 15, 2008 |
| Title |
Cap_Gata4_GR+Dkk1_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Gata4+Dkk1 injected animal cap_CHX+Dex_2.5 hours.
|
| Organism |
Xenopus laevis |
| Characteristics |
Embryo injected at 1 cell stage with Gata4-GR+Dkk1, cap cut at stage 9 and incubated with CHX and Dex, harvested after 2.5 hours.
|
| Treatment protocol |
10ng of inducible mRNA construct was injected into one cell embryos at around 20 degrees off the animal pole.
|
| Growth protocol |
Embryos were injected with 10ng of each mRNA at the one cell stage and allowed to reach stage 9, whereupon animal caps were excised and cultured in .75 NAM for 2.5 hours in the presence of cycloheximide and dexamethasone.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Xenopus leavis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Scanned using manufacturer's protocols.
|
| Description |
Embryo injected at 1 cell stage with Gata4-GR+Dkk1, cap cut at stage 9 and incubated with CHX and Dex, harvested after 2.5 hours.
|
| Data processing |
Data were normalised and analysed using Genespring 7.0 using default settings.
|
| |
|
| Submission date |
Jul 16, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Kathryn Maddison |
| Organization name |
Cardiff University
|
| Department |
Genetics
|
| Street address |
Museum Avenue
|
| City |
Cardiff |
| ZIP/Postal code |
CF14 2HB |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE8496 |
Examining the immediate early targets of Gata4 and Gata5. |
|
