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Sample GSM210751 Query DataSets for GSM210751
Status Public on Jul 15, 2008
Title Cap_Gata4_GR+Dkk1_rep4
Sample type RNA
Source name Gata4+Dkk1 injected animal cap_CHX+Dex_2.5 hours.
Organism Xenopus laevis
Characteristics Embryo injected at 1 cell stage with Gata4-GR+Dkk1, cap cut at stage 9 and incubated with CHX and Dex, harvested after 2.5 hours.
Treatment protocol 10ng of inducible mRNA construct was injected into one cell embryos at around 20 degrees off the animal pole.
Growth protocol Embryos were injected with 10ng of each mRNA at the one cell stage and allowed to reach stage 9, whereupon animal caps were excised and cultured in .75 NAM for 2.5 hours in the presence of cycloheximide and dexamethasone.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Xenopus leavis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scanned using manufacturer's protocols.
Description Embryo injected at 1 cell stage with Gata4-GR+Dkk1, cap cut at stage 9 and incubated with CHX and Dex, harvested after 2.5 hours.
Data processing Data were normalised and analysed using Genespring 7.0 using default settings.
Submission date Jul 16, 2007
Last update date Aug 14, 2011
Contact name Kathryn Maddison
Organization name Cardiff University
Department Genetics
Street address Museum Avenue
City Cardiff
ZIP/Postal code CF14 2HB
Country United Kingdom
Platform ID GPL1318
Series (1)
GSE8496 Examining the immediate early targets of Gata4 and Gata5.

Data table header descriptions

Data table
AFFX-BioB-3_at 119.5 P 0.000662
AFFX-BioB-5_at 191.9 P 0.000052
AFFX-BioB-M_at 152.6 P 0.000044
AFFX-BioC-3_at 407 P 0.000052
AFFX-BioC-5_at 403.5 P 0.000044
AFFX-BioDn-3_at 743.7 P 0.000044
AFFX-BioDn-5_at 1263.7 P 0.000044
AFFX-CreX-3_at 5385.1 P 0.000044
AFFX-CreX-5_at 7269.7 P 0.000044
AFFX-DapX-3_at 68.4 P 0.000095
AFFX-DapX-5_at 157.1 P 0.000581
AFFX-DapX-M_at 261.4 P 0.000052
AFFX-LysX-3_at 19 P 0.00011
AFFX-LysX-5_at 34.1 M 0.046482
AFFX-LysX-M_at 48.7 P 0.000052
AFFX-PheX-3_at 27.2 P 0.000044
AFFX-PheX-5_at 23.2 P 0.030976
AFFX-PheX-M_at 48.6 P 0.000225
AFFX-r2-Bs-dap-3_at 24.2 P 0.004484
AFFX-r2-Bs-dap-5_at 43.7 P 0.003595

Total number of rows: 15611

Table truncated, full table size 499 Kbytes.

Supplementary file Size Download File type/resource
GSM210751.CEL.gz 2.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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