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Sample GSM2097490 Query DataSets for GSM2097490
Status Public on Mar 01, 2019
Title Transgenic TAC 1
Sample type RNA
Source name Transgenic Heart tissue 21 Days after surgery TAC
Organism Mus musculus
Characteristics strain: C57/Bl6
genotype/variation: CnAb1 transgenic overexpressing
treatment: trans aortic banding
time: 21
Treatment protocol The heart of the mice were extracted and a section of the left ventricule was selected for RNA purification.
Growth protocol Mice were sacrificed 21 days after surgery (Sham and TAC).
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Mini Kit using DNAse treatment
Label Cy3
Label protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies)
Hybridization protocol One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies). Samples were hibridice for 17hours with 1.65μg of Cy3 at 65ºC in a hybridization oven.
Scan protocol Arrays were scanned at 5μm resolution on an Agilent DNA Microarray Scanner (G2565BA) using default setting for 4x44k format one-color arrays. Images were analyzed using Feature Extraction software version (Agilent Technologies)
Data processing Data was normaliced with RMA in GeneSpring software.
Submission date Mar 23, 2016
Last update date Mar 01, 2019
Contact name Jesus Maria Gómez-Salnero
Phone (+34) 91 4531200
Organization name CNIC
Street address Melchor Fernández Almagro, 3
City Madrid
ZIP/Postal code 28007
Country Spain
Platform ID GPL11202
Series (1)
GSE79536 The calcineurin splicing variant CnAb1 reduces pressure overload-induced cardiac hypertrophy by promoting mitohormesis

Data table header descriptions
VALUE RMA normalized signal intensity

Data table
GE_BrightCorner 0.49938822
DarkCorner -0.4838333
A_55_P1989846 -0.40373755
A_55_P1991598 0.6627064
A_55_P2022211 0.09050703
A_55_P1980764 0.30617476
A_55_P1964375 0.22938347
A_51_P128876 0.12252712
A_55_P2121042 0.15791893
A_52_P219230 -0.50968266
A_51_P207591 -0.1870606
A_55_P2131920 -0.00719595
A_55_P2404223 0.16923666
A_55_P2101944 0.38023376
A_52_P358860 0.17414713
A_51_P119031 0.14426565
A_51_P309854 -0.005209923
A_51_P343900 0.17667675
A_51_P234359 -0.045614243
A_51_P487813 -0.10537672

Total number of rows: 39485

Table truncated, full table size 967 Kbytes.

Supplementary file Size Download File type/resource
GSM2097490_B1_TAC_8C.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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