|
| Status |
Public on Mar 01, 2019 |
| Title |
Transgenic TAC 1 |
| Sample type |
RNA |
| |
|
| Source name |
Transgenic Heart tissue 21 Days after surgery TAC
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6
genotype/variation: CnAb1 transgenic overexpressing
treatment: trans aortic banding
time: 21
|
| Treatment protocol |
The heart of the mice were extracted and a section of the left ventricule was selected for RNA purification.
|
| Growth protocol |
Mice were sacrificed 21 days after surgery (Sham and TAC).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Mini Kit using DNAse treatment
|
| Label |
Cy3
|
| Label protocol |
One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies)
|
| |
|
| Hybridization protocol |
One-Color MicroArray-Based Gene Expression Analysis Protocol (Agilent Technologies). Samples were hibridice for 17hours with 1.65μg of Cy3 at 65ºC in a hybridization oven.
|
| Scan protocol |
Arrays were scanned at 5μm resolution on an Agilent DNA Microarray Scanner (G2565BA) using default setting for 4x44k format one-color arrays. Images were analyzed using Feature Extraction software version 9.5.3.1 (Agilent Technologies)
|
| Data processing |
Data was normaliced with RMA in GeneSpring software.
|
| |
|
| Submission date |
Mar 23, 2016 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Jesus Maria Gómez-Salnero |
| E-mail(s) |
jmgomez@cnic.es
|
| Phone |
(+34) 91 4531200
|
| Organization name |
CNIC
|
| Street address |
Melchor Fernández Almagro, 3
|
| City |
Madrid |
| ZIP/Postal code |
28007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE79536 |
The calcineurin splicing variant CnAb1 reduces pressure overload-induced cardiac hypertrophy by promoting mitohormesis |
|
