|
| Status |
Public on Sep 02, 2016 |
| Title |
M_truncatula_sRNA |
| Sample type |
SRA |
| |
|
| Source name |
Medicago truncatula sRNA
|
| Organism |
Medicago truncatula |
| Characteristics |
accession: F83
tissue: leaf
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue was flash frozen in liquid nitrogen for all experiments including ChIP-seq, RNA-seq and MethylC-seq. DNA was isolated using a Qiagen Plant DNeasy kit (Qiagen, Valencia, CA) following the manufacturer’s recommendations. RNA was isolated using TRIzol (Thermo Scientific, Waltham, MA) following the manufacturer’s instructions
Small RNA-seq: Libraries were constructed using the TruSeq Small RNA Library Preparation Kit (Illumina Inc).
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
small RNA sequencing of M. truncatula
|
| Data processing |
Small RNA-seq data: bed files of mapped sRNAs
genome build: A. lyrata = v1.0; A. thaliana = TAIR10; A. trichopoda = v1.0; B. distachyon = v2.1; B. oleracea = TO1000 v1.0; B. rapa = FPsc v1.3; B. vulgaris = v1.1; C. clementina = v1.0; C. rubella = v1.0; C. sativa = canSat3; C. sativus = v1.0; E. grandis = v1.1; E. salsugineum = v1.0, F. vesca = v1.1; G. max = w82.a2.v1; G. raimondii = v2.1; L. japonicus = v2.5; M. domestica = v1.0; M. esculenta = v4.1; M. guttatus = v2.0; M. truncatula = Mt4.0v1; O. sativa = v7.0; P. hallii = v0.5; P. persica = v1.0; P trichocarpa = v3.0; P. virgatum = v1.1; P. vulgaris = v1.0; R. communis = v0.1; S. bicolor = v2.1; S. lycopersicum = iTAG2.3; S. viridis = unpublished; T. cacao = V1.1; V. vinifera = GENOSCOPE.12X; Z. mays = AGPv3.21 (6a)
processed data files format and content: Small RNA-seq data: bed files of mapped sRNAs
|
| |
|
| Submission date |
Mar 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Robert J Schmitz |
| E-mail(s) |
schmitz@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
Genetics
|
| Street address |
B416 Davison Life Sciences
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30602 |
| Country |
USA |
| |
|
| Platform ID |
GPL21643 |
| Series (1) |
| GSE79526 |
Widespread natural variation of DNA methylation within angiosperms |
|
| Relations |
| BioSample |
SAMN04576070 |
| SRA |
SRX1656942 |
