|
| Status |
Public on Jun 01, 2018 |
| Title |
Z2_P53_NF1_glioma_1 |
| Sample type |
RNA |
| |
|
| Source name |
malignant glioma
|
| Organism |
Mus musculus |
| Characteristics |
genotype: hGFAP-cre;p53flox/flox;Nf1KO/+
phenotype: malignant glioma
|
| Treatment protocol |
Mice carry various genotypic configurations as indicated for each case.
|
| Growth protocol |
Cell cultures were prepared based on a previously published protocol (Wang et al., Cell 150, 816-830, 2012), and chromosome metaphase assays were performed according to an established protocol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs and genomic DNAs were extracted from wild-type control brain tissues, high-grade gliomas and MBs by using AllPrep DNA/RNA Mini Kit (Qiagen). cDNA synthesis was performed using QuantiTect Reverse Transcription Kit (Qiagen) and RT-PCR was performed using SYBR Select Master Mix (Applied Biosystems).
|
| Label |
biotin
|
| Label protocol |
Complementary RNA synthesis and labeling/hybridization/scanning of Mouse Genome 430 2.0 microarrays using GeneChip products (Affymetrix) was done as described in the GeneChip manual (see below fields).
|
| |
|
| Hybridization protocol |
Hybridization was performed following a published protocol (Miller D et al, Curr. Protoc. Hum. Genet. 2012. PMID: 18633976)
|
| Scan protocol |
GeneChips were scanned according to manufacturer recommendation
|
| Description |
phenotype: malignant glioma
|
| Data processing |
The CEL files were preprocessed and normalized by the aroma package using a custom CDF file from AffyProbeMiner (GPL9523)
|
| |
|
| Submission date |
Mar 04, 2016 |
| Last update date |
Jun 02, 2018 |
| Contact name |
Yuan Zhu |
| E-mail(s) |
YZhu@childrensnational.org
|
| Organization name |
Children's National Medical Center
|
| Street address |
111 Michigan Ave NW
|
| City |
Washington DC |
| State/province |
DC |
| ZIP/Postal code |
20010 |
| Country |
USA |
| |
|
| Platform ID |
GPL9523 |
| Series (1) |
| GSE78895 |
Rictor/mTORC2 signaling has opposing functions in adult glioma and childhood SHH medulloblastoma |
|
