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Status |
Public on Jun 01, 2018 |
Title |
Z1_Astrocytoma_3 |
Sample type |
RNA |
|
|
Source name |
malignant glioma
|
Organism |
Mus musculus |
Characteristics |
genotype: hGFAP-cre;p53KO/+;Nf1+/flox phenotype: malignant glioma
|
Treatment protocol |
Mice carry various genotypic configurations as indicated for each case.
|
Growth protocol |
Cell cultures were prepared based on a previously published protocol (Wang et al., Cell 150, 816-830, 2012), and chromosome metaphase assays were performed according to an established protocol.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs and genomic DNAs were extracted from wild-type control brain tissues, high-grade gliomas and MBs by using AllPrep DNA/RNA Mini Kit (Qiagen). cDNA synthesis was performed using QuantiTect Reverse Transcription Kit (Qiagen) and RT-PCR was performed using SYBR Select Master Mix (Applied Biosystems).
|
Label |
biotin
|
Label protocol |
Complementary RNA synthesis and labeling/hybridization/scanning of Mouse Genome 430 2.0 microarrays using GeneChip products (Affymetrix) was done as described in the GeneChip manual (see below fields).
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|
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Hybridization protocol |
Hybridization was performed following a published protocol (Miller D et al, Curr. Protoc. Hum. Genet. 2012. PMID: 18633976)
|
Scan protocol |
GeneChips were scanned according to manufacturer recommendation
|
Description |
phenotype: malignant glioma
|
Data processing |
The CEL files were preprocessed and normalized by the aroma package using a custom CDF file from AffyProbeMiner (GPL9523)
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|
|
Submission date |
Mar 04, 2016 |
Last update date |
Jun 02, 2018 |
Contact name |
Yuan Zhu |
E-mail(s) |
YZhu@childrensnational.org
|
Organization name |
Children's National Medical Center
|
Street address |
111 Michigan Ave NW
|
City |
Washington DC |
State/province |
DC |
ZIP/Postal code |
20010 |
Country |
USA |
|
|
Platform ID |
GPL9523 |
Series (1) |
GSE78895 |
Rictor/mTORC2 signaling has opposing functions in adult glioma and childhood SHH medulloblastoma |
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