|
| Status |
Public on Dec 31, 2007 |
| Title |
Expression analysis of human neuroblastoma (Affymetrix) |
| Sample type |
RNA |
| |
|
| Source name |
human neuroblastoma tissue
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: female
Age: 13 months old
Tissue: tumor adrenal grand from time-pregnant rats
Cells: neuroblastoma cells
|
| Biomaterial provider |
Hiroshima University Hospital
|
| Treatment protocol |
At surgery, right adrenal tumor and lymph node metastasis were resected. Then, small peccieces of primary tumor was picked up and frozen immediately until use.
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissue specimen using RNA isolation kit according to the manufacturer’s instructions (Stratagene, Agilent techonology, Santa Clara , CA) and purified further using the Rneasy Mini Kit (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Conversion of double stranded cDNA into biotin-labeled cRNA was accomplished using the One-Cycle cDNA Synthesis Kit (T7) (Affymetrix, Santa Clara, CA) according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
After hybridization cocktails preparation, the Human Genome U133 Plus 2.0 Array was hybridized with the fragmented labelled cRNA for 16 h at 45 °C as described in the Affymetrix Technical Analysis Manual.
|
| Scan protocol |
Scan protocol GeneChips, bound to streptavidin-phycoerythrin, were processed using the Affymetrix GeneChip Fluidics Station 450 and scanned with an GeneChip Scanner 3000 (Affymetrix).
|
| Description |
Affymetrix GeneChipTM Human Genome U133 Plus 2.0 array (~47,000 transcripts, Affymetrix, Santa Clara, CA)
|
| Data processing |
Data were processed using functional ‘ON/OFF’ state of a gene
|
| |
|
| Submission date |
Jun 27, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Eiso Hiyama |
| E-mail(s) |
eiso@hiroshima-u.ac.jp
|
| Phone |
81822575951
|
| Organization name |
Hiroshima University
|
| Department |
Natural Science Center for Basic Research and Deve
|
| Lab |
LIfe Science
|
| Street address |
1-2-3, Kasumi, Minami-ku
|
| City |
Hiroshima |
| State/province |
Hiroshima |
| ZIP/Postal code |
734-8551 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8353 |
A Mathematical Model for Affymetrix GeneChip Probe Level Data |
|
| Relations |
| Reanalyzed by |
GSE119087 |
