|
| Status |
Public on Jan 29, 2016 |
| Title |
M9 |
| Sample type |
RNA |
| |
|
| Source name |
Conjunctival whole tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Conjunctiva
dif: DIF positive
disease state: Inflamed pemphigoid
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNA extraction kit according to manufacturer's instructions. Quality control was performed with Agilent Bioanalyser at Illumina.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Inflamed pemphigoid
|
| Data processing |
Probe-level raw data was generated with GenomeStudio. It was then loaded in R and normalized using the package 'lumi'. The 'detection' function from 'lumi' was used in order to obtain the detection p-value.
|
| |
|
| Submission date |
Jan 28, 2016 |
| Last update date |
Jan 29, 2016 |
| Contact name |
Sarah Ahadome |
| E-mail(s) |
ahadome@jsei.ucla.edu
|
| Organization name |
UCLA
|
| Department |
Jules Stein
|
| Street address |
100 Stein Plaza
|
| City |
LA |
| State/province |
CA |
| ZIP/Postal code |
90095-7000 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE77361 |
Genome wide analysis of gene expression in conjunctiva whole tissue and primary conjunctival fibroblasts from patients with ocular mucous membrane pemphigoid and control patients |
|
