|
Status |
Public on Jan 29, 2016 |
Title |
UM3 |
Sample type |
RNA |
|
|
Source name |
Conjunctival whole tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: Conjunctiva dif: DIF positive disease state: Uninflamed pemphigoig
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Qiagen RNA extraction kit according to manufacturer's instructions. Quality control was performed with Agilent Bioanalyser at Illumina.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Uninflamed pemphigoig
|
Data processing |
Probe-level raw data was generated with GenomeStudio. It was then loaded in R and normalized using the package 'lumi'. The 'detection' function from 'lumi' was used in order to obtain the detection p-value.
|
|
|
Submission date |
Jan 28, 2016 |
Last update date |
Jan 29, 2016 |
Contact name |
Sarah Ahadome |
E-mail(s) |
ahadome@jsei.ucla.edu
|
Organization name |
UCLA
|
Department |
Jules Stein
|
Street address |
100 Stein Plaza
|
City |
LA |
State/province |
CA |
ZIP/Postal code |
90095-7000 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE77361 |
Genome wide analysis of gene expression in conjunctiva whole tissue and primary conjunctival fibroblasts from patients with ocular mucous membrane pemphigoid and control patients |
|