|
| Status |
Public on Jun 22, 2007 |
| Title |
R490P00325 |
| Sample type |
RNA |
| |
|
| Source name |
prostate cancer sample
|
| Organism |
Homo sapiens |
| Characteristics |
Percentage of Tumor: 43%
Percentage of Stroma: 50%
Percentage of BPH: 1%
Percentage of Atrophic Gland: 2%
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QiaGen Rneasy extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner.
|
| Description |
prostate
|
| Data processing |
The data were analyzed and normalized with RMA.
|
| |
|
| Submission date |
Jun 21, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Yipeng Wang |
| E-mail(s) |
ywang@sdibr.org
|
| Phone |
8585813960
|
| Organization name |
Vaccine Research Institute of San Diego
|
| Street address |
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE8218 |
Gene expression data from prostate cancer samples |
|
