|
| Status |
Public on May 22, 2017 |
| Title |
Scrambled siRNA 1, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Glioma cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U87MG
transfection: scrambled siRNA
|
| Treatment protocol |
100nM scrambled or siRNA against ASCL1
|
| Growth protocol |
U87MG glioma cells were grown in DMEM medium containing 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChip Scanner 3000
|
| Description |
Control RNA 48 hrs post transfection
|
| Data processing |
GCOS software
|
| |
|
| Submission date |
Jan 07, 2016 |
| Last update date |
May 22, 2017 |
| Contact name |
sainitin donakonda |
| E-mail(s) |
saigro16@gmail.com
|
| Organization name |
TUM
|
| Department |
Institute of Immunology and Experimental Oncology
|
| Lab |
Immunology
|
| Street address |
Ismaningerstr. 22
|
| City |
München |
| State/province |
Select |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16356 |
| Series (1) |
| GSE76652 |
Silencing of ASCL1 in U87MG glioma cells |
|
