|
Status |
Public on Nov 01, 2016 |
Title |
SKOV3_TR_CELL, Biological rep. 2 |
Sample type |
RNA |
|
|
Source name |
SKOV3-TR Cell
|
Organism |
Homo sapiens |
Characteristics |
cell line: SKOV3-TR tissue type: Ovarian cancer
|
Treatment protocol |
N/A
|
Growth protocol |
All cells were maintained at 37°C with 5% CO2 and 95% air.
|
Extracted molecule |
total RNA |
Extraction protocol |
Ovarian cancer cells were plated in cell culture medium containing 10% FBS and cultured for 24 h to 70% confluence. Media were replaced with 10% exosome-depleted FBS and cells were grown for 24hours more. Cells were collected by centrifugation and exosomes were isolated using supernatant with Total exosome isolation reagent (Invitrogen, Waltham, MA).
|
Label |
biotin
|
Label protocol |
Biotinylated polyA tailed RNA were prepared according to the standard Affymetrix protocol (FlashTag Biotin HSR RNA Labeling Kit, Affymetrix).
|
|
|
Hybridization protocol |
Following biotinylation, hybridization coctailed was prepared with 21.5ul labeled sample and incubated at 95C for minutes , then 45C for 5minutes. Following sample loading, arrays were loaded into trays and incibated at 48C abd 60rpm for 16 to 18hours.
|
Scan protocol |
The instructions for using the scanner can be found in Affymetrix Command Console User Guide.
|
Description |
Epithelial miRNA
|
Data processing |
The data were analyzed with Expression Console. RMA + DABG analysis was performed for the normalization.
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|
|
Submission date |
Dec 31, 2015 |
Last update date |
Nov 01, 2016 |
Contact name |
Gabriel Lopez-Berestein |
E-mail(s) |
glopez@mdanderson.org
|
Organization name |
MD Anderson Cancer Center
|
Department |
Experimental Therapeutics
|
Lab |
Lopez Lab
|
Street address |
1901 East Rd
|
City |
Houston |
State/province |
TX |
ZIP/Postal code |
77054 |
Country |
USA |
|
|
Platform ID |
GPL19117 |
Series (1) |
GSE76449 |
miRNA Expression Data from Ovarian Cancer Exosomes and Cells of Origin |
|