|
Status |
Public on Jul 01, 2007 |
Title |
Lymphoblastoid Cell Line for Individual 8172.5 log phase |
Sample type |
RNA |
|
|
Source name |
Lymphoblastoid_cell_line
|
Organism |
Homo sapiens |
Characteristics |
ped: 8172 id: 5 fatid: 1 motid: 2 sex: 1 country: UK DDAST: CONTROL rs3744350: -- rs16492: -- rs16235: -- rs16238: -- rs558317: -- rs498973: -- rs16495: -- rs12946701: -- rs16519: -- rs16522: -- rs544198: -- rs657672: -- rs657723: -- rs16527: -- rs3025203: -- rs801259: -- rs3744353: -- rs2301775: -- rs16531: -- rs521633: -- rs16530: -- rs486512: -- rs1106769: -- rs2061342: -- rs7214151: -- rs7208487: -- rs9906612: -- rs4795369: -- rs11655550: -- rs4611492: -- rs12450559: -- rs12453682: -- rs1877032: -- rs879606: -- rs9892427: -- rs1877031: -- rs2271308: -- rs931992: -- rs2941503: -- rs2941504: -- rs1565922: -- rs907092: -- rs9911688: -- rs9303277: -- rs2060941: -- rs9908694: -- rs11557467: -- rs8067378: -- rs2305480: -- rs2290400: -- rs7216389: -- rs3744246: -- rs4795405: -- rs8079416: -- rs4795408: -- rs1007654: -- rs3902025: -- rs3894194: -- rs3859192: -- rs921651: -- rs8066582: -- rs8080546: -- rs2071369: -- rs2302776: -- rs3934886: -- rs11078936: -- rs868150: -- rs7502966: -- rs1568400: -- rs939348: -- rs3744805: -- rs2071427: -- rs2269457: -- rs2071570: -- rs7211770: -- rs2280400: -- rs13706: -- rs2715553: -- rs4890109: -- rs2715556: -- rs2715555: -- rs13695: -- rs11650680: -- rs2015561: -- rs538399: -- rs7214466: -- rs8078319: -- rs749906: -- rs4890115: -- rs2290207: -- rs7214311: -- rs896136: -- rs9675320: -- rs2023906: -- rs3136685: -- rs608517: -- rs1358175: -- rs757411: -- rs11659024: -- rs1474454: -- rs1526603: -- rs2014704: -- rs2462961: -- rs2429553: -- rs1880832: -- rs9915334: -- rs9303295: -- rs1405334: -- rs4890126: -- rs4890127: -- rs2109217: -- rs8079180: -- rs2429542: -- rs975078: -- rs9898164: -- rs719867: -- rs981684: -- rs12453124: -- rs4132126: -- rs7209228: -- rs7219451: -- rs7211017: --
|
Treatment protocol |
n/a
|
Growth protocol |
Previously transformed cryo-preserved EBV cell lines were grown as 500 ml roller cultures. Once log phase had been obtained, cells were pelleted, media discarded and a mixture of RLT buffer and β-mercaptoethanol added. Pellets were vortexed to ensure thorough re-suspension after which they were frozen at -70oC and kept at -80oC.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted in using the RNeasy Maxi Kit.
|
Label |
biotin
|
Label protocol |
Procedure used standard Affymetrix labelling protocol.
|
|
|
Hybridization protocol |
Procedure used standard Affymetrix labelling protocol.
|
Scan protocol |
Procedure used standard Affymetrix labelling protocol.
|
Description |
no additional
|
Data processing |
RMA Express
|
|
|
Submission date |
Jun 08, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Goncalo Abecasis |
E-mail(s) |
goncalo@umich.edu
|
Organization name |
University of Michigan
|
Street address |
1420 Washington Heights
|
City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE8052 |
Genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |