|
| Status |
Public on Jul 01, 2007 |
| Title |
Lymphoblastoid Cell Line for Individual 8155.3 log phase |
| Sample type |
RNA |
| |
|
| Source name |
Lymphoblastoid_cell_line
|
| Organism |
Homo sapiens |
| Characteristics |
ped: 8155
id: 3
fatid: 1
motid: 2
sex: 1
country: UK
DDAST: CASE
rs3744350: --
rs16492: --
rs16235: --
rs16238: --
rs558317: --
rs498973: --
rs16495: --
rs12946701: --
rs16519: --
rs16522: --
rs544198: --
rs657672: --
rs657723: --
rs16527: --
rs3025203: --
rs801259: --
rs3744353: --
rs2301775: --
rs16531: --
rs521633: --
rs16530: --
rs486512: --
rs1106769: --
rs2061342: --
rs7214151: --
rs7208487: --
rs9906612: --
rs4795369: --
rs11655550: --
rs4611492: --
rs12450559: --
rs12453682: --
rs1877032: --
rs879606: --
rs9892427: --
rs1877031: --
rs2271308: --
rs931992: --
rs2941503: --
rs2941504: --
rs1565922: --
rs907092: --
rs9911688: --
rs9303277: --
rs2060941: --
rs9908694: --
rs11557467: --
rs8067378: --
rs2305480: --
rs2290400: --
rs7216389: --
rs3744246: --
rs4795405: --
rs8079416: --
rs4795408: --
rs1007654: --
rs3902025: --
rs3894194: --
rs3859192: --
rs921651: --
rs8066582: --
rs8080546: --
rs2071369: --
rs2302776: --
rs3934886: --
rs11078936: --
rs868150: --
rs7502966: --
rs1568400: --
rs939348: --
rs3744805: --
rs2071427: --
rs2269457: --
rs2071570: --
rs7211770: --
rs2280400: --
rs13706: --
rs2715553: --
rs4890109: --
rs2715556: --
rs2715555: --
rs13695: --
rs11650680: --
rs2015561: --
rs538399: --
rs7214466: --
rs8078319: --
rs749906: --
rs4890115: --
rs2290207: --
rs7214311: --
rs896136: --
rs9675320: --
rs2023906: --
rs3136685: --
rs608517: --
rs1358175: --
rs757411: --
rs11659024: --
rs1474454: --
rs1526603: --
rs2014704: --
rs2462961: --
rs2429553: --
rs1880832: --
rs9915334: --
rs9303295: --
rs1405334: --
rs4890126: --
rs4890127: --
rs2109217: --
rs8079180: --
rs2429542: --
rs975078: --
rs9898164: --
rs719867: --
rs981684: --
rs12453124: --
rs4132126: --
rs7209228: --
rs7219451: --
rs7211017: --
|
| Treatment protocol |
n/a
|
| Growth protocol |
Previously transformed cryo-preserved EBV cell lines were grown as 500 ml roller cultures. Once log phase had been obtained, cells were pelleted, media discarded and a mixture of RLT buffer and β-mercaptoethanol added. Pellets were vortexed to ensure thorough re-suspension after which they were frozen at -70oC and kept at -80oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted in using the RNeasy Maxi Kit.
|
| Label |
biotin
|
| Label protocol |
Procedure used standard Affymetrix labelling protocol.
|
| |
|
| Hybridization protocol |
Procedure used standard Affymetrix labelling protocol.
|
| Scan protocol |
Procedure used standard Affymetrix labelling protocol.
|
| Description |
no additional
|
| Data processing |
RMA Express
|
| |
|
| Submission date |
Jun 08, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Goncalo Abecasis |
| E-mail(s) |
goncalo@umich.edu
|
| Organization name |
University of Michigan
|
| Street address |
1420 Washington Heights
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8052 |
Genetic variants regulating ORMDL3 expression are determinants of susceptibility to childhood asthma |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
